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Chapters
- 00:05Title
- 01:21Purification of TcTERT
- 03:18T. castaneum Total RNA Isolation
- 04:53in vitro Reconstitution of T. castaneum Telomerase
- 05:38Telomerase Repeat Amplification Protocol (TRAP) Assay
- 07:57Representative Results for the in vitro Reconstitution of the Active T. castaneum Telomerase
- 08:52Conclusion
Inspanningen om de katalytische subeenheid van telomerase isoleren, tert, in voldoende hoeveelheden voor structurele studies, is voldaan met beperkt succes voor meer dan een decennium. Hier presenteren we methoden voor de isolatie van het recombinante Tribolium castaneum TERT (<em> Tc</em> TERT) en de reconstructie van de actieve<em> T. castaneum</em> Telomerase ribonucleoproteïne (RNP) complex<em> In vitro</em>.
Tags
TelomeraseT. CastaneumCatalytic SubunitIsolationRecombinantTelomerase RibonucleoproteinIn Vitro ReconstitutionDNA RepeatsTelomeresReverse TranscriptaseLinear ChromosomesEnd-to-end FusionDegradationDNA ReplicationHayflick LimitSomatic CellsCancer CellsCell ImmortalityProtein SubunitIntegral RNA ComponentTemplate SynthesisCrystal Structure
