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Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

Article DOI: 10.3791/4056-v • 14:06 min • November 12th, 2012
November 12th, 2012 •
*1, *2,3, 1,2, 1,2
1Department of Molecular Genetics, University of Toronto, 2Banting and Best Department of Medical Research, Donnelly Centre, University of Toronto, 3Department of Biochemistry, Research and Innovation Centre, University of Regina
* These authors contributed equally

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요약
Automatic Translation

Systematic, large-scale synthetic genetic (gene-gene or epistasis) interaction screens can be used to explore genetic redundancy and pathway cross-talk. Here, we describe a high-throughput quantitative synthetic genetic array screening technology, termed eSGA that we developed for elucidating epistatic relationships and exploring genetic interaction networks in Escherichia coli.

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Bacterial Functional Networks Pathways Escherichia Coli Synthetic Genetic Arrays Phenotypes Physical Interactions Functional Interactions Gene-gene Interactions Genetic Interactions GI Screens Epistatic Dependencies Functional Relationships Large-scale GI Maps Prokaryotes Functional Annotation Bacterial Genomes Quantitative Bacterial GI Screening Methods E. Coli Synthetic Genetic Array (eSGA) Screening Procedure Genome-scale Screening
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