JoVE Journal
Chemistry
Chemistry
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.
챕터
요약
We describe a simple, quantitative colorimetric assay that specifically measures the proteolytic activity of human, mouse or rat Granzyme B (GzmB). This protocol can be easily adapted for determining protease activity of other granule serine proteases by the hydrolysis of other synthetic peptide substrates with an appropriate recognition sequence.