Capturing Tissue Repair in Zebrafish Larvae with Time-lapse Brightfield Stereomicroscopy

Thomas S. Lisse; Elizabeth A. Brochu; Sandra Rieger

doi:10.3791/52654

Journal

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발생학

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시간 경과 브라이트 실체 현미경으로 Zebrafish의 애벌레에서 조직 복구를 캡처

JoVE 신문
발생학
Author Produced

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JoVE 신문 발생학

Capturing Tissue Repair in Zebrafish Larvae with Time-lapse Brightfield Stereomicroscopy

시간 경과 브라이트 실체 현미경으로 Zebrafish의 애벌레에서 조직 복구를 캡처

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10,622 Views

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14:29 min

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January 31, 2015

DOI:

10.3791/52654-v

DOI:

10.3791/52654-v

•

14:29 min

January 31, 2015

•

3 Views

Thomas S. Lisse¹, Elizabeth A. Brochu¹, Sandra Rieger¹

¹Davis Center for Regenerative Biology and Medicine,MDI Biological Laboratory

Summary

We present a protocol for capturing the dynamics of zebrafish larval tail fin regeneration on a whole-tissue scale using brightfield-based stereomicroscopy. This technique enables capturing the regeneration dynamics with single cell resolution. This methodology can be adapted to any stereomicroscope equipped with a CCD camera and time-lapse software.