G Protein-selective GPCR Conformations Measured Using FRET Sensors in a Live Cell Suspension Fluorometer Assay

Ansley Semack; Rabia U. Malik; Sivaraj Sivaramakrishnan

doi:10.3791/54696

Journal

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생물학

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G 단백질 선택적 GPCR의 입체는 라이브 셀 서스펜션 형광 분석에서 FRET 센서를 사용하여 측정

G Protein-selective GPCR Conformations Measured Using FRET Sensors in a Live Cell Suspension Fluorometer Assay

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G Protein-selective GPCR Conformations Measured Using FRET Sensors in a Live Cell Suspension Fluorometer Assay

G 단백질 선택적 GPCR의 입체는 라이브 셀 서스펜션 형광 분석에서 FRET 센서를 사용하여 측정

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9,545 Views

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09:12 min

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September 10, 2016

DOI:

10.3791/54696-v

DOI:

10.3791/54696-v

•

09:12 min

September 10, 2016

•

8 Views

Ansley Semack¹, Rabia U. Malik², Sivaraj Sivaramakrishnan¹

¹Genetics, Cell Biology, and Development,University of Minnesota, ²Department of Cell and Developmental Biology,University of Michigan

내레이션 대본

Summary

Simple methods to detect the selective activation of G proteins by G protein-coupled receptors remain an outstanding challenge in cell signaling. Here, Fӧrster resonance energy transfer (FRET) biosensors have been developed by pairwise tethering a GPCR to G protein peptides to probe conformational changes at controlled concentrations in live cells.