CRISPR-Mediated Reorganization of Chromatin Loop Structure

Stefanie L. Morgan; Erin Y. Chang; Natasha C. Mariano; Abel Bermudez; Nicole L. Arruda; Fanting Wu; Yunhai Luo; Gautam Shankar; Star K. Huynh; Chiao-Chain Huang; Sharon J. Pitteri; Kevin C. Wang

doi:10.3791/57457

Journal

/

유전학

/

CRISPR-Mediated Reorganization of Chromatin Loop Structure

JoVE 신문
유전학

This content is Free Access.

JoVE 신문 유전학

CRISPR-Mediated Reorganization of Chromatin Loop Structure

12,416 Views

•

09:20 min

•

September 14, 2018

DOI:

10.3791/57457-v

DOI:

10.3791/57457-v

•

09:20 min

September 14, 2018

•

25 Views

Stefanie L. Morgan*^1,2, Erin Y. Chang*¹, Natasha C. Mariano¹, Abel Bermudez³, Nicole L. Arruda⁴, Fanting Wu⁵, Yunhai Luo¹, Gautam Shankar¹, Star K. Huynh¹, Chiao-Chain Huang⁵, Sharon J. Pitteri³, Kevin C. Wang^1,2,6

¹Department of Dermatology, Program in Epithelial Biology,Stanford University School of Medicine, ²Program in Cancer Biology,Stanford University School of Medicine, ³Canary Center for Cancer Early Detection, Department of Radiology,Stanford University School of Medicine, ⁴Department of Biology,Bridgewater State University, ⁵System Biosciences, ⁶Veterans Affairs Healthcare System

내레이션 대본

Summary

Chromatin looping plays a significant role in gene regulation; however, there have been no technological advances that allow for selective and reversible modification of chromatin loops. Here we describe a powerful system for chromatin loop re-organization using CRISPR-dCas9 (CLOuD9), demonstrated to selectively and reversibly modulate gene expression at targeted loci.