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Biochemistry

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JoVE Journal Biochemistry
Korrekturlæsning og DNA reparation analyse ved hjælp af Single Nucleotide udvidelse og MALDI-TOF-massespektrometri analyse
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Korrekturlæsning og DNA reparation analyse ved hjælp af Single Nucleotide udvidelse og MALDI-TOF-massespektrometri analyse

Article DOI: 10.3791/57862-v • 11:08 min • June 19th, 2018
June 19th, 2018 •
*1,2, *3, 1, 1, 1, 2, 1,2, 1,2, 1,2
1Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan University, 2Department of Laboratory Medicine, National Taiwan University Hospital, 3Center for Microbial Pathogenesis, Nationwide Children's Hospital and the Department of Pediatrics, The Ohio State University
* These authors contributed equally

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En ikke-mærket, ikke-radio-isotopiske metode for DNA polymerase korrekturlæsning og en DNA reparation assay blev udviklet ved hjælp af høj opløsning MALDI-TOF-massespektrometri og en enkelt nukleotid udvidelse strategi. Analysen viste sig at være meget specifikke, enkel, hurtig og let at udføre for korrekturlæsning og reparation patches kortere end 9-nukleotider.

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Biokemi sag 136 DNA polymerase korrekturlæsning DNA reparation MALDI-TOF-massespektrometri enkelt nucleotid udvidelse dideoxyribonucleotide trifosfat DNA uoverensstemmelse DNA replikation fejl
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