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En høj overførselshastighed Luciferase Assay at evaluere proteolyse af Single-omsætning Protease PCSK9

Article DOI: 10.3791/58265-v • 08:15 min • August 28th, 2018
August 28th, 2018 •
1, 2, 2
1Division of Cardiology, Department of Medicine, Zuckerberg San Francisco General and University of California San Francisco, 2Department of Cellular and Molecular Pharmacology and Howard Hughes Medical Institute, University of California San Francisco

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Denne protokol udgør en metode til at vurdere de proteolytiske aktivitet af et uløseligt lav-aktivitet, enkelt omsætning protease i cellulære sammenhæng. Specifikt, kan denne metode anvendes til at vurdere de proteolytiske aktivitet af PCSK9, en vigtig drivkraft for lipid metabolisme hvis proteolytiske aktivitet er nødvendig for dens ultimative hypercholesterolemic funktion.

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Biokemi spørgsmålet 138 Proprotein convertase subtilisin/kexin type 9 protease substrat specificitet høj overførselshastighed assay luciferase low-density lipoprotein low-density lipoprotein receptor enkelt nukleotid polymorfisme stof screening
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