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En hög genomströmning luciferas analysen att utvärdera proteolys av enda-omsättning proteas PCSK9

Article DOI: 10.3791/58265-v • 08:15 min • August 28th, 2018
August 28th, 2018 •
1, 2, 2
1Division of Cardiology, Department of Medicine, Zuckerberg San Francisco General and University of California San Francisco, 2Department of Cellular and Molecular Pharmacology and Howard Hughes Medical Institute, University of California San Francisco

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Detta protokoll presenterar en metod för att utvärdera den proteolytiska aktiviteten av en inneboende lågaktivt, enda omsättning proteashämmare i cellulära sammanhang. Specifikt, används denna metod för att utvärdera den proteolytiska aktiviteten av PCSK9, en viktig drivkraft i lipidmetabolismen vars proteolytiska verksamhet krävs för dess ultimata hypercholesterolemic funktion.

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Biokemi fråga 138 Proprotein convertase subtilisin/kexin type 9 proteashämmare substrat specificitet hög genomströmning assay luciferas low-density lipoprotein LDL-receptorn enda nukleotid polymorfism drug screening
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