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Journal / Biology / 利用两色单分子荧光在原位杂交中Escherichia coli探索大肠杆菌中空间和时间中的…
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利用两色单分子荧光在原位杂交中Escherichia coli探索大肠杆菌中空间和时间中的 mRNA 动力学
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利用两色单分子荧光在原位杂交中Escherichia coli探索大肠杆菌中空间和时间中的 mRNA 动力学

Article DOI: 10.3791/61520-v • 10:01 min • July 30th, 2020
July 30th, 2020 •
1,2,3,4, 1,2
1Department of Physics, University of Illinois at Urbana-Champaign, 2Center for the Physics of Living Cells, University of Illinois at Urbana-Champaign, 3Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, 4Center for Biophysics and Quantitative Biology, University of Illinois at Urbana-Champaign

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该协议描述了单分子荧光原位杂交（smFISH）用于测量mRNA合成和降解的体内动力学的应用。

Tags

生物学， 问题 161， smFISH， RNA 成像， mRNA 定位， 单细胞， 单分子， 转录， 转录伸长， 过早终止， mRNA 降解
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