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Tredimensjonal in vitro biomimetisk modell av neuroblastom ved bruk av kollagenbaserte stillas
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Tredimensjonal in vitro biomimetisk modell av neuroblastom ved bruk av kollagenbaserte stillas

Article DOI: 10.3791/62627-v • 07:48 min • July 9th, 2021
July 9th, 2021 •
*1,2,3, *1,2,3, 4, 3,5,6, 1,2,3,6,7
1Cancer Bioengineering Group, Department of Anatomy and Regenerative Medicine, RCSI University of Medicine and Health Sciences, Dublin, Ireland, 2School of Pharmacy and Biomolecular Sciences, RCSI University of Medicine and Health Sciences, Dublin, Ireland, 3Tissue Engineering Research Group, Department of Anatomy and Regenerative Medicine, RCSI University of Medicine and Health Sciences, Dublin, Ireland, 7Department of Chemistry, Royal College of Surgeons in Ireland (RCSI), 123 St. Stephen's Green, Dublin 2, Ireland, 5Trinity Centre for Bioengineering, Trinity College Dublin, Dublin, Ireland, 6Advanced Materials and Bioengineering Research Centre (AMBER), RCSI and TCD, Dublin, Ireland, 7National Children’s Research Centre, Our Lady's Children's Hospital Crumlin, Dublin, Ireland
* These authors contributed equally

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Dette papiret viser trinnene som kreves for å frø neuroblastomcellelinjer på tidligere beskrevne tredimensjonale kollagenbaserte stillas, opprettholde cellevekst for en forhåndsbestemt tidsramme og hente stillas for flere cellevekst- og celleadferdsanalyser og nedstrømsapplikasjoner, tilpasningsdyktige for å tilfredsstille en rekke eksperimentelle mål.

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Tredimensjonal in vitro biomimetisk modell neuroblastom kollagenbaserte stillaser barnekreft tumorvev ekstracellulær matriks (ECM) sykdomsprogresjon pasientprognose terapeutisk respons neuroblastomcellelinjer nanohydroksyapatitt (nHA) glykosaminoglykaner (GAGs) bein og benmarg metastatiske steder porøs struktur cellefeste spredning migrasjon celleklynger terapeutika in vivo-situasjon stillasbasert kultursystem todimensjonal (2D) celle Kultur DNA-kvantifisering celle levedyktighet
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