세포 신호를 분석하는 통합 툴킷: 힘, 동작, 형태학 및 형광

5.2K 조회수

7회 인용됨

14:55 분

2022년 3월 5일

10.3791/63095-v

2022년 3월 5일

5.2K 조회수

Alyson Nguyen^*¹ , Keith Battle^*²^,³ , Sunita S. Paudel^*²^,³ , Ningyong Xu² , Jessica Bell³ , Linn Ayers³ , Cassandra Chapman⁴ , Ajay P. Singh⁵ , Srinivas Palanki⁶ , Thomas Rich³^,⁷ , Diego F. Alvarez⁸ , Troy Stevens²^,³ , Dhananjay T. Tambe³^,⁴^,⁷

¹Biomedical Sciences, Pat Capps Covey College of Allied Health Professions, University of South Alabama, ²Department of Physiology and Cell Biology, College of Medicine, University of South Alabama, ³Center for Lung Biology, College of Medicine, University of South Alabama, ⁴William B. Burnsed Jr. Mechanical, Aerospace, and Biomedical Engineering Department, College of Engineering, University of South Alabama, ⁵Mitchell Cancer Institute, College of Medicine, University of South Alabama, ⁶Department of Chemical and Biomedical Engineering, West Virginia University, ⁷Department of Pharmacology, College of Medicine, University of South Alabama, ⁸Department of Physiology & Pharmacology, College of Osteopathic Medicine, Sam Houston State University

^* These authors contributed equally

iTACS(셀룰러 신호)를 분석하는 통합 툴킷은 부착 셀에서 다양한 화학 및 기계적 신호를 동시에 측정하는 과정을 자동화합니다. iTACS는 지역 사회 기반의 개발을 용이하게하고 연구원이 교육 배경에 관계없이 모든 플랫폼 기능을 사용할 수 있도록 설계되었습니다.

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Chapters in this video

0:02

Introduction

1:46

Creation of Position List

3:57

Automated Image Acquisition for the Remaining Experiment

4:34

Setting Up Automated Data Analysis

6:08

Quantification of Hydrogel Deformation

6:37

Quantification of Cell-ECM and Cell-cell Forces

8:44

Segmentation of the Individual Cells in the Images

9:47

Mapping of the Pixel Intensities on the Cells

11:06

Mapping of the Forces and Motion on the Cells

11:51

Tracking of the Cellular Indentities across the Experiment

12:51

Generation of Time Tracks of the Assessed Cellular Properties

13:27

Generation of the Heat Maps of the Assessed Cellular Properties

13:53

Representative Results

14:30

Conclusion