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Bioengineering

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Engineered Lung Tissues Prepared from Decellularized Lung Slices

Article DOI: 10.3791/63151-v • 08:01 min • January 21st, 2022
January 21st, 2022 •
1,2, 1, 1,3, 1,4
1Department of Biomedical Engineering, Yale University, 2Yale School of Medicine, 3Department of Cellular and Molecular Physiology, Yale School of Medicine, 4Department of Anesthesiology, Yale School of Medicine

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요약
Automatic Translation

This protocol describes a method to generate reproducible, small-scale engineered lung tissues, by repopulating decellularized precision-cut lung slices with alveolar epithelial type 2 cells, fibroblasts, and endothelial cells.

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Engineered Lung Tissues Decellularized Lung Slices Tissue Culture Substrate Moderate Throughput Flexible Platform Tissue Scaffolds Cells Culture Media Agarose HBSS Trachea Cannula Lung Lobes Petri Dish Solidify Agarose Lung Slices Protocol 100-millimeter Petri Dish PBS Frozen Slices Thawing Process Fine Forceps Razor Blade
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