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用于高内涵筛选和分析应用的大规模生产球体的稳健方法
JoVE 신문
생물학
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JoVE 신문 생물학
A Robust Method for the Large-Scale Production of Spheroids for High-Content Screening and Analysis Applications
DOI:

06:40 min

December 28, 2021

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Chapters

  • 00:05Introduction
  • 00:35Coating 96-Well Plate with ECM Basement Material
  • 01:07Subculturing and Cell Counting
  • 01:52Seeding Cells into ECM Basement Material-Coated Plates
  • 02:41Image Acquisition of Spheroids
  • 03:07Image Acquisition of Spheroids
  • 04:35Results: Observation and Imaging of Spheroids
  • 06:07Conclusion

Summary

자동 번역

该协议详细介绍了生产三种不同类型的球体的方法,使其适用于大规模高内涵筛选和分析。此外,还提供了示例,展示了如何在球体和单个细胞水平上分析它们。

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