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Journal / Biology / 一种用于评估细胞DNA损伤的高通量彗星检测方法…
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一种用于评估细胞DNA损伤的高通量彗星检测方法

Article DOI: 10.3791/63559-v • 07:57 min • May 10th, 2022
May 10th, 2022 •
*1, *2, *3, 4, 5, 1
1Oxidative Stress Group, Dept. Cell Biology, Microbiology and Molecular Biology, College of Arts and Sciences, University of South Florida, 2Cepheid (Danaher Corp.), US Technical Operations, 3Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, 4Department of Chemistry and Biochemistry, Institute of Environment, Florida International University, 5Leicester School of Allied Health Sciences, Faculty of Health and Life Sciences, De Montfort University
* These authors contributed equally

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彗星测定是检测DNA损伤的常用方法。本研究描述了一种在彗星测定的代表性变体中运行载玻片的方法。这种方法显著增加了样品数量，同时减少了分析运行时间、玻片操作次数和凝胶损坏风险。

Tags

生物学，第183期，彗星测定，DNA损伤，DNA修复，氧化应激，生物标志物，电泳
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