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Journal / Neuroscience / 人小胶质细胞样细胞：使用人突触体从诱导多能干细胞和 体外…
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JoVE Journal Neuroscience
人小胶质细胞样细胞：使用人突触体从诱导多能干细胞和 体外 活细胞吞噬测定中分化
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人小胶质细胞样细胞：使用人突触体从诱导多能干细胞和 体外 活细胞吞噬测定中分化

Article DOI: 10.3791/64323-v • 11:19 min • August 18th, 2022
August 18th, 2022 •
1,2, 1
1Department of Neurology, University of Massachusetts Chan Medical School, 2Translation Science Program, Morningside Graduate School of Biomedical Sciences, University of Massachusetts Chan Medical School

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该协议描述了人诱导多能干细胞（iPSC）成小胶质细胞样细胞进行 体外 实验的分化过程。我们还包括从iPSC衍生的下运动神经元生成人突触体的详细程序，该神经元可用作使用活细胞成像系统进行 体外 吞噬测定的底物。

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神经科学，第186期，
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