High-Quality Seizure-Like Activity from Acute Brain Slices Using a Complementary Metal-Oxide-Semiconductor High-Density Microelectrode Array System

Melissa L. Blotter; Isaac W. Stubbs; Jacob H. Norby; Maxwell Holmes; Ben Kearsley; Alexis Given; Kutter Hine; Micah R. Shepherd; R. Ryley Parrish

doi:10.3791/67065

Complementary Metal-Oxide-Semiconductor High-Density Microelectrode Array System을 사용한 급성 뇌...

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Neuroscience

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JoVE Journal Neuroscience

High-Quality Seizure-Like Activity from Acute Brain Slices Using a Complementary Metal-Oxide-Semiconductor High-Density Microelectrode Array System

Complementary Metal-Oxide-Semiconductor High-Density Microelectrode Array System을 사용한 급성 뇌 절편에서 고품질 발작 유사 활동

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06:28 min

September 27, 2024

DOI: 10.3791/67065-v

Melissa L. Blotter*^1,2, Isaac W. Stubbs*^1,2, Jacob H. Norby*^1,2, Maxwell Holmes^1,2, Ben Kearsley³, Alexis Given¹, Kutter Hine^1,4, Micah R. Shepherd⁵, R. Ryley Parrish^1,2

¹Department of Cell Biology and Physiology,Brigham Young University, ²Neuroscience Center,Brigham Young University, ³Department of Statistics,Brigham Young University, ⁴Department of Biology,Brigham Young University, ⁵Department of Physics and Astronomy,Brigham Young University

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Overview

This study outlines a protocol for using complementary metal-oxide-semiconductor high-density microelectrode array systems (CMOS-HD-MEAs) to investigate seizure-like activity from ex vivo brain slices. The research aims to enhance the understanding of seizure initiation, propagation, and termination, with a focus on developing novel therapies for status epilepticus.

Key Study Components

Area of Science

Neuroscience
Electrophysiology
Epileptology

Background

Seizures can have complex initiation and propagation mechanisms.
Status epilepticus is a critical condition that often resists treatment.
High-density microelectrode arrays provide detailed electrophysiological data.
Different paradigms offer insights into the dynamics of seizure-like activity.

Purpose of Study

To establish a reliable protocol for recording seizure activity.
To explore the spatial and temporal patterns of status epilepticus.
To inform the development of more effective treatments for severe seizures.

Methods Used

The main platform used is CMOS-HD-MEAs with ex vivo brain slices.
Brain slices are subjected to various treatment paradigms to investigate seizure-like activity.
Detailed preparation and maintenance steps are provided for optimal recordings.
The protocol includes steps for preparing the microelectrode array and ensuring proper environmental conditions.
Electrophysiological activity is recorded to analyze seizure characteristics.

Main Results

Neocortical regions show significant electrographic seizure-like activity under specific conditions.
Hippocampal regions exhibited variability in seizure-like activity.
Different paradigms revealed distinct power dynamics in seizure frequencies.

Conclusions

This study facilitates a deeper understanding of seizure mechanisms using advanced technology.
The findings may contribute to identifying new therapeutic targets for epilepsy treatment.
The research emphasizes the importance of spatial and temporal analysis in understanding seizure activity.

Frequently Asked Questions

What are the advantages of using CMOS-HD-MEAs?

CMOS-HD-MEAs allow for high-resolution recordings of electrophysiological data, providing detailed insights into brain activity and seizure dynamics.

How is seizure activity recorded in this study?

Seizure activity is recorded from brain slices using CMOS-HD-MEAs, which capture local field potentials during various treatment paradigms.

What types of data can be obtained from the CMOS-HD-MEAs?

The technology enables the recording of high-resolution local field potentials, facilitating analysis of seizure patterns and dynamics in the brain.

How can the method be applied to other research?

This protocol can be adapted for studying various neurological disorders by altering the treatment paradigms applied to the brain slices.

Are there any limitations to using this approach?

Some limitations may include the variability between brain slices and the need for precise experimental conditions to ensure consistent results.

여기에서는 상보적 금속 산화물 반도체 고밀도 미세전극 어레이 시스템(CMOS-HD-MEAs)을 사용하여 생체 외 뇌 절편에서 발작과 같은 활동을 기록하는 프로토콜을 간략하게 설명합니다.

Parrish 연구실에서는 발작이 어떻게 시작되고, 전파되고, 끝나는지 이해하는 데 관심이 많습니다. 우리는 특히 발작이 저절로 끝나지 않는 생명을 위협하는 상태인 간질에 대한 새로운 치료법을 탐구하는 데 관심이 있습니다. 우리는 연구에 CMOS 고밀도 미세 전극 어레이 시스템을 사용합니다.

이러한 첨단 기술을 통해 뇌 절편에서 고해상도 전기생리학적 데이터를 기록하여 상세한 국소자기장 전위를 캡처할 수 있습니다. 이것은 발작 패턴과 같은 복잡한 뇌 활동을 공간적, 시간적 정밀도로 이해하는 데 도움이 됩니다. 앞으로 우리는 종종 항간질제에 내성이 생기는 장기간의 발작 상태인 간질의 상태의 공간적, 시간적 전파 패턴을 탐구할 계획입니다.

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