Iterative Bleaching Extends Multiplicity with Use of Staining Automation for Core Facilities

Anna E. Tseng; Aoife O'Connell; Aaron Benhamou; Shivraj Yabaji; Igor Kramnik; Nicholas A. Crossland; Emily Speranza

doi:10.3791/67853

JoVE Journal
Immunology and Infection

This content is Free Access.

JoVE Journal Immunology and Infection

Iterative Bleaching Extends Multiplicity with Use of Staining Automation for Core Facilities

반복적 표백은 핵심 시설에 염색 자동화를 사용하여 다양성을 확장합니다.

Full Text

605 Views

04:52 min

August 6, 2025

DOI: 10.3791/67853-v

Anna E. Tseng^1,2, Aoife O'Connell¹, Aaron Benhamou^1,3, Shivraj Yabaji^1,2, Igor Kramnik^1,2, Nicholas A. Crossland^1,2,3, Emily Speranza⁴

¹National Emerging Infectious Diseases Laboratories (NEIDL),Boston University, ²Department of Pathology and Laboratory Medicine,Boston University Chobanian and Avedisian School of Medicine, ³Department of Virology, Immunology and Microbiology,Boston University Chobanian and Avedisian School of Medicine, ⁴Florida Research and Innovation Center,Cleveland Clinic Foundation

Please note that some of the translations on this page are AI generated. Click here for the English version.

여기에서는 상업적으로 이용 가능한 자동 조직 염색기를 활용하는 IBEX(Iterative Bleaching Extends Multiplicity) 방법의 적용과 티라마이드 신호 증폭 기반 시약 사용에 대한 최적화 고려 사항을 설명합니다.

Explore More Videos

면역학 및 감염 222호