The fascinating epitranscriptome comprises a diverse and dynamic landscape of RNA modifications that critically regulate RNA processing, stability, and translation, with profound implications in development, homeostasis, and disease. However, its complexity, marked by numerous modification types, structural versatility, and context-dependent functions, poses substantial challenges for systematic mapping and functional interpretation. A major bottleneck remains the lack of robust, standardized technologies for precise detection, quantification, and mechanistic elucidation of these modifications.

This topical Collection on epitranscriptomics aims to address these methodological gaps by gathering cutting-edge advances in experimental and computational approaches. We invite submissions that detail refined experimental workflows and practical techniques for mapping, quantifying, and functionally validating RNA modifications across diverse organisms and systems. These may include novel sequencing strategies, mass spectrometry, chemical probing, structural analysis, and AI-driven tools etc. The papers published in this collection will serve as a practical resource to foster technical reproducibility, accelerate the discovery of novel modifications, and bridge epitranscriptomic research with therapeutic innovation across the fields.