Commercial Kits
Commercial Kits - Related Videos
09:40
Analysis of the c-KIT Ligand Promoter Using Chromatin Immunoprecipitation
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2017
DNA-protein interactions are essential for multiple biological processes. During the evaluation of cellular functions, the analysis of DNA-protein interactions is indispensable for understanding gene regulation. Chromatin immunoprecipitation (ChIP) is a powerful tool to analyze such interactions in vivo.
07:06
Mouse Sperm Cryopreservation and Recovery using the I·Cryo Kit
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Cited by 5 •
2011
Here we demonstrate the newly developed I•Cryo kit for mouse sperm cryopreservation. Two-cell stage embryo development with frozen-thawed sperm was improved consistently in 5 mouse strains with the use of this kit. Over a 1.5 year period, 49 genetically modified mouse lines were archived by sperm cryopreservation with the I•Cryo kit and later successfully recovered by IVF.
06:51
High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits
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Cited by 5 •
2016
A high-throughput assay for enzyme screening is described. This multiplexed ready-to-use assay kit comprises of pre-chosen Chromogenic Polymer Hydrogel (CPH) substrates and complex Insoluble Chromogenic Biomass (ICB) substrates. Target enzymes are polysaccharide degrading endo-enzymes and proteases.
09:00
Use of the Protease Fluorescent Detection Kit to Determine Protease Activity
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Cited by 15 •
2009
The Protease Fluorescent Detection Kit is designed for the measurement of protease activity using fluorometry. It is also suitable for detection of trace amounts of protease contamination. The method is based on the proteolytic hydroysis of a proprietary formulation of a FITC-labeled casein substrate.
11:03
Pouring and Running a Protein Gel by reusing Commercial Cassettes
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Cited by 2 •
2012
Our protocol demonstrates how to pour multiple protein gels at a time by recycling Invitrogen Nupage Novex minigel cassettes, and inexpensive materials purchased at a home improvement store. This economical and streamlined method includes a way to store the gels at 4°C for a few weeks. By re-using the plastic gel cassettes from commercially available gels, labs that run frequent protein gels can save significant costs and help the environment.
FAQs
Commercial kits improve assay consistency by supplying reaction components that have been selected and combined for a defined target, enzyme, or sample type. The user applies specified conditions, including pH, temperature, and incubation time, so the intended chemical interaction proceeds within a controlled range. This reduces variation caused by preparing reagents independently and supports more comparable results across experiments.
Controls help distinguish a meaningful assay response from variation introduced by the procedure or sample. Included control materials are processed under the same specified conditions as experimental samples, creating a reference for judging whether the reaction and readout behaved consistently. This is especially important when comparing results across routine analyses or method-development experiments.
Spectrophotometric, fluorometric, and electrophoretic readouts translate an assay reaction or prepared sample into an observable result, but they do not provide identical measurement formats. The selected readout should match the kit’s intended target and the available analysis workflow. Recognizing the readout helps researchers interpret results and assess whether the kit fits their experiment.
Detection limits indicate the range in which an assay can meaningfully identify or measure its target, while compatibility concerns whether the sample works with the supplied reaction conditions. Checking both factors before use helps prevent a technically consistent procedure from producing unsuitable results for the intended protein, nucleic acid, enzyme, or metabolite analysis.
A typical workflow follows the supplied protocol, combines the provided reaction components with the relevant biological sample, and applies the specified pH, temperature, and incubation conditions. Researchers also process the supplied controls and then use the designated spectrophotometric, fluorometric, or electrophoretic readout. This structured sequence limits reagent preparation and procedural variation.
Commercial kits support several routine biochemical tasks, including protein quantification, nucleic acid purification, enzyme activity measurements, and metabolite detection. Their standardized components and protocols allow researchers to focus on the target measurement or preparation rather than independently optimizing every reagent. The resulting readout can support routine analysis, comparisons, and further method development.
They are especially useful when researchers need a consistent starting procedure for routine analysis or method development. Because the reagents and protocol are standardized, investigators can evaluate the target assay while reducing variation from independent reagent preparation. They must still assess detection limits, sample compatibility, and controls to determine whether the approach suits the intended application.