Tau Circular Rna
Tau Circular Rna - Related Videos
08:25
Identification of Circular RNAs using RNA Sequencing
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Cited by 30 •
2019
Circular RNAs (circRNAs) are non-coding RNAs that may have roles in transcriptional regulation and mediating interactions between proteins. Following assessment of different parameters for construction of circRNA sequencing libraries, a protocol was compiled utilizing stranded total RNA library preparation with RNase R pre-treatment and is presented here.
02:19
Lentiviral-Mediated Transduction of Human Neurons for Tau Protein Aggregation Analysis
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2025
This video demonstrates the process of transducing human neuronal cells with a lentivirus encoding a mutant human tau protein tagged with yellow fluorescent protein (YFP) to study protein aggregation. It outlines the steps from viral entry to mutant tau expression, culminating in the formation of visible cytoplasmic tau aggregates in the neurons.
11:37
Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism
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Cited by 5 •
2017
This article provides a detailed procedure on the solid-phase synthesis, purification, and characterization of dodecamers of RNA modified at the C2'-O-position. UV-vis and circular dichroism photometric analyses are used to quantify and characterize structural aspects, i.e., single-strands or double-strands.
09:22
In Vitro Aggregation Assays Using Hyperphosphorylated Tau Protein
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Cited by 21 •
2015
Unmodified and hyperphosphorylated tau proteins were used in two in vitro aggregation assays to reveal the hyperphosphorylation-dependent fast aggregation kinetics. These assays pave the way for future screens for compounds that can modulate the propensity of hyperphosphorylated tau to form fibrils that underlie the progression of Alzheimer’s disease.
01:16
Kendall's Tau Test
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2025
Kendall's tau test, also known as the Kendall rank coefficient test, is a nonparametric method for assessing association between two variables. This test is particularly useful for identifying significant correlations when the distributions of the sample and population are unknown. Developed in 1938 by the British statistician Sir Maurice George Kendall, the tau coefficient (denoted as τ) serves as a rank correlation coefficient, with values ranging from -1 to +1. A τ value of +1 indicates that...
FAQs
The key rearrangement occurs when a downstream splice donor joins an upstream splice acceptor rather than following the usual linear splicing arrangement. This back-splicing reaction connects separated transcript regions into a covalently closed circle. The resulting structure provides a biochemical basis for studying how MAPT-derived RNA differs from conventional transcript products.
A covalently closed RNA circle lacks the free 5′ and 3′ ends that characterize linear RNA molecules. This structural property often makes it more resistant to exonucleases, enzymes that degrade RNA from exposed ends. That stability is important when investigating persistence, post-transcriptional regulation, or whether circular molecules could remain detectable in biochemical and disease-related studies.
The circular and linear forms differ primarily in transcript architecture rather than in their association with the MAPT gene. A closed circle can show greater resistance to exonuclease degradation, while its circular configuration may influence interactions with RNA-binding proteins and post-transcriptional regulatory processes. Comparing these forms can clarify how RNA structure contributes to tau-related regulation.
RNA-binding proteins may recognize or associate with features of the circular transcript, making them relevant to post-transcriptional regulation. Biochemical studies can examine these interactions to determine whether they alter the behavior or regulatory significance of MAPT-derived circles. This perspective connects RNA structure with possible effects on tau expression and, ultimately, neuronal function.
A focused investigation would examine how the molecule is produced through back-splicing, whether its RNA remains covalently closed, and how that structure relates to exonuclease resistance. Researchers can then consider interactions with RNA-binding proteins and possible effects on tau expression. These features connect molecular architecture with functional questions without treating the circle as equivalent to linear MAPT RNA.
Their covalently closed architecture and often increased resistance to exonucleases make these transcripts relevant to studies of RNA persistence and detection. Researchers can investigate whether MAPT-derived circles provide information associated with Alzheimer’s disease or related tauopathies. Such work supports biomarker development while also helping distinguish disease-associated signals from broader RNA-regulatory mechanisms.
They provide a molecular system for connecting RNA processing with tau biology. At the biochemical level, researchers can relate back-splicing, RNA stability, and RNA-binding-protein interactions to possible changes in tau expression or neuronal function. In disease research, this framework is relevant to Alzheimer’s disease and related tauopathies, where mechanistic studies and biomarker development address complementary questions.