Bio-afm Automation
Bio-afm Automation - Related Videos
08:21
Quantitative Hardness Measurement by Instrumented AFM-indentation
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Cited by 6 •
2016
This experimental protocol describes how atomic force microscopy can be used to measure hardness at the true nanometer scale and to detect single atomistic plasticity events.
09:47
AFM and Microrheology in the Zebrafish Embryo Yolk Cell
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Cited by 3 •
2017
The lack of tools to measure material properties and tensional parameters in vivo prevents validating their roles during development. We employed atomic force microscopy (AFM) and nanoparticle-tracking to quantify mechanical features on the intact zebrafish embryo yolk cell during epiboly. These methods are reliable and widely applicable avoiding intrusive interventions.
09:27
Automation of Bio-Atomic Force Microscope Measurements on Hundreds of C. albicans Cells
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Cited by 3 •
2021
This protocol aims to automate AFM measurements on hundreds of microbial cells. First, microbes are immobilized into PDMS stamp microstructures and then force spectroscopy measurements are performed automatically on hundreds of immobilized cells.
10:34
Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
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Cited by 2 •
2010
This procedure describes a quick and easy workflow to introduce siRNA into difficult to transfect cell lines and follow gene expression by real-time PCR. Use of an automated cell counter, multi-well electroporation plate, and automated electrophoresis station provide quick and reliable results without the need for expensive robotic handling.
16:38
Bridging the Bio-Electronic Interface with Biofabrication
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Cited by 3 •
2012
This article describes a biofabrication approach: deposition of stimuli-responsive polysaccharides in the presence of biased electrodes to create biocompatible films which can be functionalized with cells or proteins. We demonstrate a bench-top strategy for the generation of the films as well as their basic uses for creating interactive biofunctionalized surfaces for lab-on-a-chip applications.
FAQs
Automation connects probe positioning, surface scanning, force spectroscopy, and image acquisition within a predefined workflow. The instrument can move between these operations in a planned sequence rather than relying on separate manual adjustments for every measurement. This coordination helps maintain consistent measurement routines and supports more reproducible comparisons among biological samples, surfaces, or experimental conditions.
Force spectroscopy enables the instrument to examine how biological systems respond to applied forces and contributes quantitative information about mechanical properties. When incorporated into an automated workflow, these measurements can be collected alongside surface scans and images. That combination links nanoscale structure with mechanical behavior, which is useful for studying cells, membranes, biomaterials, and molecular interactions.
Liquid conditions and applied forces are experimental variables that can influence measurements of biological systems. Automated control helps keep these conditions consistent while the instrument performs scanning, force spectroscopy, or image acquisition. Controlling them is especially relevant when comparing samples, because differences in the measurement environment could otherwise complicate interpretation of nanoscale morphology or mechanical properties.
The main distinction is how consistently the measurement sequence is executed. Manual operation can require repeated operator decisions for probe positioning, scanning, force application, and image acquisition, whereas automation applies predefined instructions. Reducing operator variability can improve consistency and reproducibility, making it easier to standardize experiments and compare results across biological samples or repeated measurements.
A workflow can begin with positioning the probe relative to the biological sample, followed by programmed surface scanning and force spectroscopy. The system then acquires images and records the resulting measurements under specified conditions, such as a liquid environment or selected applied forces. Using a predefined sequence allows these operations to proceed as a coordinated experimental routine.
The core setup requires a biological atomic force microscope, a probe, a sample surface, and programmed instrument control. Depending on the experiment, the system also manages liquid environments, applied forces, scanning operations, and image acquisition. These components allow researchers to collect nanoscale morphology and mechanical information while maintaining a defined and repeatable measurement procedure.
In bioengineering, automated measurements can characterize cell surfaces, membrane structures, biomaterials, and molecular interactions. The resulting nanoscale morphology and mechanical-property data support biomaterial design, cell-mechanics studies, and disease research. Automation also contributes to more scalable and standardized experimental methods, helping researchers conduct measurements with less operator variability and greater consistency.