Footprint-free Ipsc
Footprint-free Ipsc - Related Videos
09:47
Spotting Cheetahs: Identifying Individuals by Their Footprints
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Cited by 25 •
2016
The cheetah (Acinonyx jubatus) is an iconic, endangered species, but conservation efforts are challenged by habitat shrinkage and conflict with commercial farmers. The footprint identification technique, a robust, accurate and cost-effective image classification system, is a new approach to monitoring cheetahs.
09:16
Efficient Generation and Editing of Feeder-free IPSCs from Human Pancreatic Cells Using the CRISPR-Cas9 System
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Cited by 4 •
2017
This protocol describes in detail the generation of footprint-free induced pluripotent stem cells (iPSCs) from human pancreatic cells in feeder-free conditions, followed by editing using CRISPR/Cas9 ribonucleoproteins and characterization of the modified single-cell clones.
02:49
Assessing Gait by Footprint Analysis: A Method to Test Motor Coordination in Mice
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2023
In this video, we describe the footprint testing procedure and related analysis to assess gait and motor coordination in mice.
13:41
Monitoring Equilibrium Changes in RNA Structure by 'Peroxidative' and 'Oxidative' Hydroxyl Radical Footprinting
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Cited by 4 •
2011
This protocol describes how to quantify the Mg(II)-dependent formation of RNA tertiary structure by two methods of hydroxyl radical footprinting.
03:29
PiggyBac Transposon-Mediated Gene Editing in Human iPSCs: A Procedure to Integrate Gene of Interest in Human iPSCs Using PiggyBac Transposon System
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2025
This video demonstrates the procedure to generate motor neurons by transfection of human iPSCs with PiggyBac transposon system by the ectopic expression of lineage-specific transcription factors.
FAQs
Transient delivery makes reprogramming-factor activity temporary rather than genome-persistent. During the period when the factors are expressed, they can reset a differentiated cell toward pluripotency. Afterward, the delivery materials are cleared or degraded, so the resulting iPSC population is not designed to retain permanent vector sequences. This separation supports controlled reprogramming.
The key distinction is the fate of the delivery system after reprogramming. Footprint-free iPSC generation relies on disappearance or degradation of introduced materials, whereas permanent vector sequences remain in the genome. This difference reduces the risk of unintended genomic modification and becomes important when researchers need to interpret cell behavior without a persistent vector sequence as an additional genetic variable.
Avoiding permanent genomic footprints provides bioengineers with a more controlled starting cell population. This matters because disease models, drug evaluations, engineered tissues, and regenerative strategies depend on interpreting cell behavior without adding persistent vector sequences as unwanted genetic variables. The resulting control strengthens studies designed to support clinically relevant cell-based therapies.
A conceptual workflow begins with differentiated cells, followed by transient delivery of reprogramming factors. The factors are allowed to act while they reset the cellular state, and the delivery materials are subsequently cleared or degraded. The resulting footprint-free iPSCs can then provide starting material for downstream bioengineering studies, rather than serving only as the endpoint of reprogramming.
These cells provide a flexible starting material for constructing disease models and evaluating drugs. Because the reprogramming process is designed to avoid permanent vector sequences, researchers can conduct downstream studies with reduced concern that persistent delivery-related genetic changes will complicate interpretation. This supports more controlled comparisons of cellular behavior in disease-related or pharmacological investigations.
In tissue engineering and regenerative medicine, footprint-free iPSCs can serve as an adaptable source for developing cell-based strategies. Their reduced risk of unintended genomic modification supports tighter control over studies involving engineered tissues and potential therapeutic development. This characteristic is especially relevant when researchers seek cell products and procedures with stronger potential for clinical translation.