Hollow Fiber Bioreactor
Hollow Fiber Bioreactor - Related Videos
08:28
Hollow Fiber Bioreactors for In Vivo-like Mammalian Tissue Culture
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Cited by 28 •
2016
The functional behavior of cells in culture can be improved by culturing in more in vivo-like 3-dimensional culture environments16-21. This manuscript describes the set-up and operation of a hollow fiber bioreactor system for in vivo-like mammalian tissue culture.
09:09
Large-Scale Production of Extracellular Vesicles from High-Density 3D Cell Cultures in Hollow Fiber Perfusion Bioreactors
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2026
Instructions are provided for setting up and using a 3D perfusion hollow fiber bioreactor for extracellular vesicle production from MSC and other cell types. This protocol describes a method for producing extracellular vesicles at high concentrations on a continuous basis while controlling cell density in hollow fiber bioreactors.
06:01
Production of Extracellular Matrix Fibers via Sacrificial Hollow Fiber Membrane Cell Culture
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Cited by 2 •
2019
The goal of this protocol is production of whole extracellular matrix fibers targeted for wound repair which are suitable for preclinical evaluation as part of a regenerative scaffold implant. These fibers are produced by the culture of fibroblasts on hollow fiber membranes and extracted by dissolution of the membranes.
12:54
Operation of a Benchtop Bioreactor
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Cited by 9 •
2013
Fermentors are used to increase culture yield and productivity of bioengineered cells. After screening multiple microbial or animal cell culture candidates in shake flasks, the next logical step is to increase the selected culture’s biomass with the fermentor. This video demonstrates the setup and operation of a typical benchtop bioreactor system.
12:11
A Modified EPA Method 1623 that Uses Tangential Flow Hollow-fiber Ultrafiltration and Heat Dissociation Steps to Detect Waterborne Cryptosporidium and Giardia spp.
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Cited by 11 •
2012
This protocol describes the use of a tangential flow hollow-fiber ultrafiltration sample concentration system and a heat dissociation as alternative steps for the detection of waterborne Cryptosporidium and Giardia species using EPA Method 1623.
FAQs
The semipermeable fiber walls separate the medium pathway from the cell compartment while permitting exchange between them. Nutrients and oxygen diffuse toward cells, and metabolic wastes move away from the cell-containing region. This arrangement supports continuous environmental exchange without requiring the cells to occupy the same physical space as the flowing medium.
Perfusion continuously supplies fresh culture medium and helps remove metabolic wastes, maintaining conditions suitable for prolonged growth. The bundled fibers also provide a large surface area for mass transfer, meaning movement of nutrients, oxygen, and waste across the membrane. Together, these features help sustain higher cell densities than many conventional culture arrangements.
A hollow fiber bioreactor can expose cells to a three-dimensional environment with continuous exchange across fiber membranes, whereas conventional two-dimensional culture grows cells on a flatter surface. The three-dimensional arrangement may more closely resemble tissue conditions and supports prolonged cultivation, making it useful when spatial organization and sustained mass transfer matter.
Medium can flow through the fiber lumens or around the outer fiber surfaces, while exchange occurs across the semipermeable walls. This configuration determines which region carries the medium and which region contains the cells, but both arrangements support delivery of nutrients and oxygen and removal of wastes through diffusion. The selected orientation is therefore part of system design.
A basic setup requires a bundle of semipermeable hollow fibers, a cell-containing compartment, and a culture-medium pathway. Researchers establish medium flow either through the fiber lumens or around the fiber surfaces, then maintain controlled cultivation conditions that support diffusion and cell growth. The resulting configuration enables ongoing nutrient delivery and waste removal during operation.
This system is useful when experiments require high-density cell growth, prolonged cultivation, or conditions that resemble tissue more closely than flat culture. In bioengineering, those requirements arise in biologics production, tissue-engineering research, and studies needing scalable cell cultivation. Its perfusion-based design connects sustained operation with controlled exchange across a large membrane area.
Hollow fiber bioreactors provide a platform for maintaining dense cell populations while supplying nutrients and oxygen and moving metabolic wastes away from the cell compartment. Depending on the study, this supports production of biologics, investigation of tissue-engineering environments, and prolonged cell-culture experiments. The system is particularly relevant when researchers need controlled, scalable cultivation rather than short-term growth.