Human Engineered Cardiac Tissue
Human Engineered Cardiac Tissue - Related Videos
11:51
Construction of Defined Human Engineered Cardiac Tissues to Study Mechanisms of Cardiac Cell Therapy
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Cited by 16 •
2016
This manuscript describes the creation of defined engineered cardiac tissues using surface marker expression and cell sorting. The defined tissues can then be used in a multi-tissue bioreactor to investigate mechanisms of cardiac cell therapy in order to provide a functional, yet controlled, model system of the human heart.
10:09
Capillary Force Lithography for Cardiac Tissue Engineering
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Cited by 27 •
2014
In this protocol, we demonstrate the fabrication of biomimetic cardiac cell culture substrata made from two distinct polymeric materials using capillary force lithography. The described methods provide a scalable, cost-effective technique to engineer the structure and function of macroscopic cardiac tissues for in vitro and in vivo applications.
10:18
Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering
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Cited by 79 •
2011
We describe the isolation of neonatal cardiomyocytes and the preparation of the cells for encapsulation in fibrin hydrogel constructs for tissue engineering. We describe methods for analyzing the tissue engineered myocardium after the culture period including active force generated upon electrical stimulation and cell viability and immunohistological staining.
10:39
Automated Contraction Analysis of Human Engineered Heart Tissue for Cardiac Drug Safety Screening
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Cited by 38 •
2017
Here, we show the generation of human engineered heart tissue from induced pluripotent stem cells (hiPSC)-derived cardiomyocytes. We present a method to analyze contraction force and exemplary alteration of contraction pattern by the hERG channel inhibitor E-4031. This method shows high level of robustness and suitability for cardiac drug screening.
11:12
Tissue Engineering of a Human 3D in vitro Tumor Test System
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Cited by 34 •
2013
Methods to create human 3D tumor tissues as test systems are described. These technologies are based on a decellularized Biological Vascularized Scaffold (BioVaSc), primary human cells and a tumor cell line, which can be cultured under static as well as under dynamic conditions in a flow bioreactor.
FAQs
These cues promote different features needed for cardiac function, including cardiomyocyte alignment, maturation, and coordinated contraction. Their use makes the laboratory model more closely reproduce important structural and functional properties of native myocardium. Because the conditions can be controlled, researchers can examine how specific environmental signals influence tissue organization and contractile physiology.
Biomaterial scaffolds and hydrogels provide a three-dimensional setting in which human cardiomyocytes can be combined and cultured. This environment supports the development of tissue structures that reproduce selected features of heart muscle rather than relying only on isolated cells. Their inclusion therefore helps create a controllable model for studying cardiac structure and function.
Cell alignment contributes to the organized structure needed for coordinated contraction. Culture conditions that promote alignment and maturation help the engineered tissue reproduce key functional characteristics of myocardium, allowing researchers to investigate contractile physiology in a more structured model. This organization is especially relevant when examining how engineered tissue behaves as a collective rather than as separate cardiomyocytes.
Controlled culture allows researchers to study cardiac behavior under defined biochemical, mechanical, or electrical conditions. By regulating these cues, investigators can examine relationships among tissue organization, cardiomyocyte maturation, and contraction. The resulting platform supports focused studies of cardiac development, normal contractile physiology, and disease mechanisms within a laboratory-constructed human tissue model.
A general workflow begins by combining human cardiomyocytes with a biomaterial scaffold or hydrogel. The construct is then cultured under selected biochemical, mechanical, or electrical cues that encourage alignment, maturation, and coordinated contraction. These steps produce a three-dimensional tissue model that can be used for controlled investigation of cardiac structure, function, and disease-related behavior.
Researchers can use the model when they need a controllable three-dimensional human platform that reproduces important structural and functional features of myocardium. It supports studies of cardiac development, contractile physiology, and disease mechanisms while also enabling drug screening and toxicity testing. Its value comes from connecting cellular behavior with organized tissue-level contraction.
For drug research, the tissue provides a human cardiac model for screening compounds and evaluating potential toxicity in a controlled setting. In bioengineering, it helps advance strategies for cardiac repair and may inform future regenerative therapies. These applications extend the model beyond basic physiology by linking engineered tissue behavior to therapeutic development and repair-oriented research.