Crispr-cas9 Genome Editing
Crispr-cas9 Genome Editing - Related Videos
01:28
CRISPR/Cas9 Genome Editing
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2025
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
08:31
Genome Editing
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2023
A well-established technique for modifying specific sequences in the genome is gene targeting by homologous recombination, but this method can be laborious and only works in certain organisms. Recent advances have led to the development of “genome editing”, which works by inducing double-strand breaks in DNA using engineered nuclease enzymes guided to target genomic sites by either proteins or RNAs that recognize specific sequences. When a cell attempts to repair this damage, mutations can be...
02:58
CRISPR-Mediated Base Editing Tools: A Genome Editing Technique to Induce Targeted Base Substitution
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2025
This video explains the concept of CRISPR-mediated cytosine base editors for inducing targeted nucleotide substitution.
05:12
All-in-One CRISPR Genome Editing: A Method for Homology Directed Repair-Based Gene Knock-In in Cultured Cells Using CRISPR-Cas9 System
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2025
In this video, we demonstrate all-in-one CRISPR-Cas9 based genome editing in cultured cells where Cas9 and sgRNA are provided as a single plasmid construct to the cells. The CRISPR-Cas9 system and desired gene to be inserted was introduced in cells through electroporation technique to facilitate successful gene editing.
04:22
A Technique for Gene Editing in Natural Killer Cells Using CRISPR Cas9
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2025
This video demonstrates a technique for Cas9 ribonucleoprotein-mediated genetic modification of primary natural killer (NK) cells. A Cas9 ribonucleoprotein, consisting of a Cas9 endonuclease bound to a guide RNA (gRNA) formed by base pairing a CRISPR RNA (crRNA) and a trans-activating crRNA (tracrRNA), is introduced into primary natural killer cells via electroporation. The ribonucleoprotein targets and cleaves the host DNA at the target site, leading to gene knockout via modification of the...
FAQs
The guide RNA provides sequence-matching information that brings Cas9 to a selected DNA region, but targeting also depends on a nearby PAM sequence. Cas9 cuts both DNA strands near that adjacent site. Together, guide-RNA complementarity and PAM availability provide the recognition framework that makes the system programmable for different genomic targets.
The repair pathway strongly influences the result. Error-prone non-homologous end joining can generate changes useful for disrupting a gene, supporting knockout experiments. Homology-directed repair uses a supplied repair template to guide a more specific sequence modification. Researchers therefore select or interpret the editing design according to whether loss of function or sequence replacement is the goal.
Editing strategy shapes the biological question. A knockout can help reveal what happens when a gene’s function is disrupted, whereas a targeted sequence modification can examine the effect of a defined variant or correction. This distinction lets CRISPR-Cas9 support functional genomics and disease-focused studies without treating every edit as equivalent.
A typical workflow identifies a DNA sequence that can be matched by a guide RNA and lies near an appropriate PAM, then brings Cas9 to that region. After the nuclease creates the double-strand break, the cell repairs it through non-homologous end joining or, if provided, homology-directed repair using a template. The resulting DNA change is then interpreted in light of the chosen repair route.
By making targeted changes in selected genes, the method allows researchers to connect DNA sequence with biological function. Gene knockouts can test the consequences of disrupting a gene, while precise modifications can examine particular sequence changes. These experiments make CRISPR-Cas9 useful for functional genomics, including investigations of development and disease-associated variants.
Beyond gene-function analysis, the technology supports disease modeling and therapeutic development, including research on disease-associated variants. Its programmability also extends its use into agriculture and biotechnology. These applications reflect a common advantage: researchers can direct experiments toward selected DNA sequences while comparing the biological consequences of disruption or more specific modification.