Genetic Strains
Genetic Strains - Related Videos
09:52
Genetic Manipulation in Δku80 Strains for Functional Genomic Analysis of Toxoplasma gondii
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Cited by 19 •
2013
Here we report a method for using type I and type II Δku80 strains of Toxoplasma gondii to efficiently generate targeted gene deletions and gene replacements for functional genomic analysis.
02:58
Measurement of Compressive Stress-Strain Response at Small-Strains
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2025
This protocol presents the configuration of a compression test device capable of precisely measuring the mechanical properties of microstructures in the small-strain region, along with a systematic method for compression testing using this device. The proposed device can be used to analyze various microstructures and the mechanical behavior of polymer-based materials.
08:45
Genetic Crosses
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2023
To dissect genetic processes or create organisms with novel suites of traits, scientists can perform genetic crosses, or the purposeful mating of two organisms. The recombination of parental genetic material in the offspring allows researchers to deduce the functions, interactions, and locations of genes. This video will examine how genetic crosses were influential in developing Mendel's three laws of inheritance, which form the basis of our understanding of genetics. One genetic crossing...
09:13
Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy
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Cited by 33 •
2011
The reverse genetics system for the Rift Valley fever virus MP-12 vaccine strain is a useful tool for creating additional MP-12 mutants with increased attenuation and immunogenicity. We describe the protocol to generate and characterize NSs mutant strains.
08:50
Genetic Screens
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2023
Genetic screens are critical tools for defining gene function and understanding gene interactions. Screens typically involve mutating genes and then assessing the affected organisms for phenotypes of interest. The process can be “forward”, where mutations are generated randomly to identify unknown genes responsible for the phenotypes, or it can be “reverse”, where specific genes are targeted for mutation to observe what phenotypes are produced.Here, JoVE reviews various types of genetic...
FAQs
Comparative strain analysis links inherited differences to biological effects by examining how contrasting genetic backgrounds correspond to different phenotypes. If strains differ in a trait under the same experimental conditions, the comparison can suggest that genetic variation contributes to that outcome. This approach helps investigate gene function and relationships between genotype, phenotype, and biological performance.
Controlled maintenance reduces non-genetic variation that could obscure differences between strains. Keeping a strain under consistent conditions allows researchers to attribute observed phenotypic changes more confidently to inherited background rather than uncontrolled environmental differences. Standardization also makes results easier to reproduce across experiments, strengthening comparisons among studies that examine physiology, development, disease mechanisms, or treatment responses.
Genetic effects cannot be interpreted independently of experimental conditions. Comparing strains under environmental conditions or treatments can show whether inherited differences are associated with different responses. These comparisons can identify relationships between genetic background and response to a condition or treatment. Interpreting the outcome therefore requires attention to both the inherited traits and the environment or intervention applied.
An investigation typically begins by selecting strains with defined genetic backgrounds or distinguishing inherited traits, then maintaining them under controlled conditions. Researchers next compare relevant phenotypes across the strains, with consistent conditions applied to support a meaningful comparison. The resulting differences can be examined in relation to genotype, environmental exposure, or treatment.
Genetic strains support studies across multiple biological areas, including gene function, disease mechanisms, development, physiology, and responses to environmental conditions or treatments. Their value is not limited to describing differences between organisms. By connecting inherited variation with measurable biological outcomes, strain-based experiments can help researchers investigate how genetic background contributes to normal processes or disease-related characteristics.
Strains may be generated or distinguished through mutation, recombination, natural variation, or selective breeding. These routes create inherited differences that researchers can maintain and study as a defined background. The choice of strain therefore affects what comparison is possible: one design may focus on a naturally occurring difference, whereas another may examine traits produced through breeding or genetic change.