Gonad Extrusion Technique
Gonad Extrusion Technique - Related Videos
02:59
C. elegans Gonad Extrusion: A Rapid Dissection Technique
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2023
This video describes a method to dissect gonads from adult C. elegans hermaphrodites, resulting in tissues that are suitable for immunostaining and fluorescent imaging.
10:43
Dissection of Larval Zebrafish Gonadal Tissue
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Cited by 6 •
2017
Here, we present a protocol for isolating gonadal tissue of larval zebrafish, which will facilitate investigations of zebrafish sex differentiation and maintenance.
10:22
Demonstration of the Power Law Model Through Extrusion
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2023
Source: Kerry M. Dooley and Michael G. Benton, Department of Chemical Engineering, Louisiana State University, Baton Rouge, LA Polymer melts are often formed into simple shapes or "extrudates", such as cylindrical pellets, flat sheets, or pipe, using an extruder.1 Polyolefins are among the most common extrudable polymers. Extrusion involves transporting and melting solid feed, which is sometimes mixed with non-polymeric materials, and the pressure build-up and transport of the melt or mixture.
03:59
Gonad Microinjection: A Method of Compound Delivery Directly into the Germline of C. elegans
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2023
This video describes microinjection, a common method of creating transgenic C. elegans strains. In the example, we will see microinjection used to introduce a ribonucleoprotein (RNP) complex for CRISPR-bases gene editing.
09:39
Live Imaging of Cell Extrusion from the Epidermis of Developing Zebrafish
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Cited by 20 •
2011
Dying cells are extruded from epithelial tissues by concerted contraction of neighboring cells without disrupting barrier function. The optical clarity of developing zebrafish provides an excellent system to visualize extrusion in living epithelia. Here we describe methods to induce and image extrusion in the larval zebrafish epidermis at cellular resolution.
FAQs
Controlled pressure helps release the gonad through the opening while reducing mechanical damage to its cellular organization. Excessive or poorly directed force could compromise the tissue available for examination, whereas gentle extrusion preserves structures needed for microscopy, staining, and morphological assessment. This balance is central to obtaining a specimen that remains informative after removal from the intact organism.
Maintaining cellular organization allows investigators to distinguish gonadal structures and relate individual cell types to developmental stages. Preserved tissue can support microscopic examination and staining, making structural differences easier to interpret. It also improves the value of comparisons involving gonad development, gametogenesis, or changes associated with genetic and environmental conditions, because observed patterns are less likely to reflect dissection damage.
The exposed tissue can be evaluated for cellular organization, cell-type distribution, and developmental-stage features through microscopy and staining. Investigators may also analyze changes in gene expression, extending the method beyond visual inspection. These readouts connect tissue structure with reproductive processes and can help assess how genetic or environmental conditions influence gonadal development and function.
The specimen is first immobilized to stabilize the body and limit unintended movement. A controlled incision or opening is then made to provide access, followed by gentle pressure that releases the gonad while minimizing tissue disruption. After extrusion, the tissue may be mounted for microscopy, stained for cellular identification, or prepared for analyses of morphology and gene expression.
Extruded tissue can be mounted directly for microscopic examination, allowing investigators to inspect its organization and developmental features. Staining can help identify cell types or distinguish developmental stages, while additional analysis can assess morphology or gene expression. The appropriate readout depends on the research question, but each uses the newly accessible tissue to obtain information from the gonad itself.
This approach is useful when researchers need direct access to reproductive tissue rather than relying only on whole-organism imaging. It supports investigations of gonad development and gametogenesis, as well as studies of reproductive biology. Because the tissue can be examined after exposure to genetic or environmental conditions, the method also enables assessment of associated cellular and structural changes.