Gfp Fluorescence Detection
Gfp Fluorescence Detection - Related Videos
03:05
Fluorescence-Detection Size-Exclusion Chromatography: A Technique to Identify the Integrity of Fluorescent Membrane Proteins upon Detergent Solubilization
0 Views •
2025
In this video, we demonstrate a fluorescence-detection size-exclusion chromatography technique to screen for green fluorescent protein (GFP)-fused membrane protein stability in Escherichia coli following detergent solubilization. Proteins exhibiting a symmetrical peak in the size-exclusion profile with little or no free GFP indicate purified proteins with minimum degradation and aggregation, which can subsequently be selected for structure-function analysis.
03:24
Establishing Experimental Metastases Mouse Model: Implanting GFP Expressing CRC Organoid Cells in PDX Model to Detect Micrometastases
0 Views •
2023
This video describes the protocol for generating metastases by the PDX derived CRC organoids labeled with GFP lentivirus into the spleen of an immunodeficient mouse. Cancer micrometastases colonizing other organs can be detected using a fluorescence microscope to assess the GFP expression.
05:08
Fluorescent Orthotopic Mouse Model: Implanting GFP Expressing Cancer Cells into Mouse for Assessing Tumor Progression In Vivo
0 Views •
2023
This video demonstrates the protocol for implanting green fluorescent protein-expressing pancreatic cancer cells orthotopically into the pancreas of the mouse. The orthotopic mouse model helps in investigating the efficacy of novel therapeutics on primary and metastatic tumors.
03:44
Detection of Inflammasome Activation via Fluorescence Microscopy
0 Views •
2025
This video demonstrates a method to detect NLRP3 inflammasome activation in macrophages using fluorescent reporter probes. The cells are treated with potassium ionophores and glycine to induce inflammasome assembly and caspase-1 activation. Fluorescent reporter probes visualize activated caspase-1, and cells are stained with a fluorescent dye for nuclei visualization.
14:16
Fluorescence detection methods for microfluidic droplet platforms
0 Views •
Cited by 20 •
2011
Droplet-based microfluidic platforms are promising candidates for high throughput experimentation since they are able to generate picoliter, self-compartmentalized vessels inexpensively at kHz rates. Through integration with fast, sensitive and high resolution fluorescence spectroscopic methods, the large amounts of information generated within these systems can be efficiently extracted, harnessed and utilized.
FAQs
The signal depends on exciting GFP with blue or ultraviolet light and detecting the green light released afterward. Its chromophore absorbs the incoming energy and emits light at a different wavelength, allowing fluorescence instruments to distinguish the reporter signal from the excitation source. Measurement therefore links optical excitation with detectable reporter output.
A GFP tag can be associated with cells, proteins, or molecular constructs, so its placement determines the biological feature being visualized. In cancer research, the resulting signal may help reveal where tumor cells are located, where a protein is present, or when a gene-associated activity occurs. This connects fluorescence patterns with specific cellular processes.
Fluorescence microscopes detect GFP spatially, making them useful for viewing labeled cells, proteins, and cellular interactions. Plate readers measure fluorescence from samples in a plate, supporting numerical comparisons across experimental conditions. Both detect GFP emission, but their outputs emphasize different forms of information: visual localization versus broader quantitative measurement.
A typical workflow begins with cells or molecular constructs carrying GFP, followed by illumination with blue or ultraviolet light. The emitted green fluorescence is then collected with a fluorescence microscope or plate reader and measured. Researchers can compare these signals among conditions or follow them over time to evaluate changes in the system.
The method is useful when researchers need to track tumor-cell behavior, monitor gene expression, assess experimental treatments, or evaluate disease models. GFP-tagged cells and constructs provide a measurable way to follow these processes, helping connect visible or quantified fluorescence changes with cancer-related biological responses and experimental outcomes.
Because the approach enables noninvasive visualization, researchers can monitor labeled cells or molecular activity over time rather than relying only on a single endpoint. Repeated fluorescence measurements can help reveal tumor progression, treatment response, or changing cellular interactions within a disease model, providing a time-based view of experimental behavior.