Bioorthogonal Labeling
Bioorthogonal Labeling - Related Videos
10:54
Preparation and Evaluation of 99mTc-labeled Tridentate Chelates for Pre-targeting Using Bioorthogonal Chemistry
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Cited by 7 •
2017
Here, we describe a protocol for radiolabeling and in vivo testing of tridentate 99mTc(I) chelate-tetrazine derivatives for pre-targeting and bioorthogonal chemistry.
06:46
Imaging Glycans in Zebrafish Embryos by Metabolic Labeling and Bioorthogonal Click Chemistry
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Cited by 11 •
2011
A click-chemistry based method that allows for the rapid, noninvasive, and robust labeling of alkyne-tagged glycans in zebrafish embryos is described. Fucosylated glycans in the enveloping layer of zebrafish embryos in the late gastrulation stage were imaged in this study.
08:28
Metabolic Labeling
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2023
Metabolic labeling is used to probe the biochemical transformations and modifications that occur in a cell. This is accomplished by using chemical analogs that mimic the structure of natural biomolecules. Cells utilize analogs in their endogenous biochemical processes, producing compounds that are labeled. The label allows for the incorporation of detection and affinity tags, which can then be used to elucidate metabolic pathways using other biochemical analytical techniques, such as SDS-PAGE...
12:31
Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry
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Cited by 14 •
2012
Adenovirus particles are engineered to contain either the unnatural amino acid analogue azidohomoalanine or the azido sugar O-GlcNAz. The azide group of each is chemoselectively ligated via "click" chemistry reactions as a means of viral surface modification.
09:19
Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy
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Cited by 3 •
2025
This protocol presents a step-by-step guide for the Simultaneous Label-free Autofluorescence Multi-harmonic (SLAM) microscopic technique, including details on how to generate the laser light source, prepare a tissue sample, conduct imaging, and analyze the data. SLAM advances nonlinear microscopy by measuring four complementary label-free contrasts to investigate the tissue microenvironment.
FAQs
The labeling reaction must recognize the introduced functional groups while largely ignoring naturally occurring cellular compounds. This selectivity helps preserve native biochemical processes, so observed fluorescence, affinity capture, or imaging signals are more likely to reflect the tagged biomolecule rather than chemical disruption caused by the labeling strategy.
Two representative partner classes are azides with alkynes and tetrazines with strained alkenes. Selecting between them provides different chemical options for connecting an introduced functional group with a detection tag, while maintaining the central requirement that the reaction proceed selectively in the biological environment.
Reactions used for Bioorthogonal Labeling must proceed rapidly under physiological conditions, because the target molecules remain within living cells or organisms. Compatibility with this environment helps limit interference with normal biochemistry and supports labeling during studies of molecular localization, movement, signaling, and distribution.
Researchers can connect fluorescent dyes, affinity tags, or imaging probes to the introduced chemical handles. Fluorescent and imaging tags support visualization and localization, whereas affinity tags enable detection through selective capture or analysis. The chosen tag therefore determines how the labeled biomolecule will be observed or tracked.
A typical strategy introduces a compatible functional group into the biomolecule or biological system, then uses a selective reaction to attach a chosen detection tag. The resulting labeled target can be examined for its presence, location, or movement, allowing the workflow to connect chemical modification with a measurable biological outcome.
The strategy can be applied to proteins, glycans, lipids, and nucleic acids. By tagging these classes, researchers can investigate molecular dynamics, cell signaling, disease mechanisms, and drug distribution in cells or organisms. This breadth makes the approach useful across chemical biology and biomedical research rather than for a single biomolecule type.