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06:46 min
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June 06, 2011
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Summary
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A click-chemistry based method that allows for the rapid, noninvasive, and robust labeling of alkyne-tagged glycans in zebrafish embryos is described. Fucosylated glycans in the enveloping layer of zebrafish embryos in the late gastrulation stage were imaged in this study.
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Cite this Article
Jiang, H., Feng, L., Soriano del Amo, D., Seidel III, R. D., Marlow, F., Wu, P. Imaging Glycans in Zebrafish Embryos by Metabolic Labeling and Bioorthogonal Click Chemistry. J. Vis. Exp. (52), e2686, doi:10.3791/2686 (2011).
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