Epr Imaging Experiment
Epr Imaging Experiment - Related Videos
11:07
Electron Paramagnetic Resonance (EPR) Spectroscopy
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2023
Source: David C. Powers, Tamara M. Powers, Texas A&M In this video, we will learn the basic principles behind Electron Paramagnetic Resonance (EPR). We will use EPR spectroscopy to study how dibutylhydroxy toluene (BHT) behaves as an antioxidant in the autoxidation of aliphatic aldehydes.
06:01
EPR Monitored Redox Titration of the Cofactors of Saccharomyces cerevisiae Nar1
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Cited by 6 •
2014
The goal of this protocol is to use electron paramagnetic resonance (EPR) monitored redox titrations to identify different cofactors of Saccharomyces cerevisiae Nar1. Redox titrations offer a very robust way to obtain midpoint potentials of different redox active cofactors in enzymes and proteins.
11:19
Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels
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Cited by 7 •
2016
This article describes methods for site-directed spin labeling and reconstitution of pentameric ligand-gated channels for Electron Paramagnetic Resonance studies. This protocol can be adapted for any membrane protein. The reconstitution method described here can also be used for patch-clamp measurements of macroscopic and single-channel currents in a defined lipid system.
10:17
Guidelines and Experience Using Imaging Biomarker Explorer (IBEX) for Radiomics
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Cited by 28 •
2018
We describe IBEX, an open-source tool designed for medical imaging radiomics studies, and how to use this tool. In addition, some published works that have used IBEX for uncertainty analysis and model building are showcased.
10:32
Image-based Lagrangian Particle Tracking in Bed-load Experiments
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Cited by 14 •
2017
The manuscript presents a protocol for the conduction of bed-load sediment transport experiments where the moving particles are tracked by image analysis. The experimental facility, the procedures for run realization and data processing, and finally some proof-of-concept results are presented here.
FAQs
The gradients make the resonance condition depend on location. When the applied magnetic field and microwave excitation interact with paramagnetic species, spins at different positions contribute signals at position-dependent resonance conditions. Computational reconstruction interprets those encoded signals and converts them into a spatial map, allowing the distribution of unpaired electrons to be examined rather than only detected as a bulk signal.
An image can carry information about more than where a signal occurs. Because the resonance signals also reflect the chemical environment of unpaired electrons, the reconstructed spatial pattern can be considered alongside local chemical differences. This makes the experiment useful for connecting the distribution of a radical or transition-metal ion with the chemistry occurring in different parts of a sample.
The magnetic field establishes the condition under which electron spins can respond, while microwave excitation supplies the stimulus for detecting that response. Changing the field in a controlled way and introducing gradients makes the resonance signal position-dependent. Together, these elements provide the raw spatially encoded information needed for computational reconstruction of the chemical map.
The workflow begins with a sample containing paramagnetic species. The experiment applies a magnetic field and microwave excitation, then uses controlled field gradients to acquire signals whose resonance conditions vary with position. Computational reconstruction follows the measurement, transforming those signals into an image of the species distribution and its associated chemical information.
Chemists can apply it to measure where free radicals or transition-metal ions occur, follow changes during reaction processes, and characterize catalysts or materials. It can also assess oxygen or redox conditions. These uses are valuable when spatially resolved information is needed to determine how paramagnetic species are distributed across a sample.
The method links molecular spin behavior to spatially resolved chemical information. A reconstructed pattern can show where paramagnetic species are concentrated, while the associated resonance response provides information about their chemical environment. In chemistry, that combination supports interpretation of radical behavior, transition-metal-containing systems, catalytic materials, and reaction-related changes within a sample.