Kelly Clamp Dissection
Kelly Clamp Dissection - Related Videos
09:57
Dissection of 6.5 dpc Mouse Embryos
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Cited by 28 •
2007
Isolation of postimplantation-stage embryos allows one to study gene patterning and analyze cell-lineage decision making processes during embryonic development, but proper dissection of the early embryo can be challenging. This protocol describes a method for isolating early primitive-streak-stage embryos (~6.5 days post coitum [dpc]).
09:38
Dissecting Mechanoenzymatic Properties of Processive Myosins with Ultrafast Force-Clamp Spectroscopy
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Cited by 1 •
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Presented here is a comprehensive protocol to perform ultrafast force-clamp experiments on processive myosin-5 motors, which could be easily extended to the study of other classes of processive motors. The protocol details all the necessary steps, from the setup of the experimental apparatus to sample preparation, data acquisition and analysis.
09:43
Patch Clamp Electrophysiology
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2023
Neuron cell membranes are populated with ion channels that control the movement of charge into and out of the cell, thereby regulating neuron firing. One extremely useful technique for investigating the biophysical properties of these channels is called patch clamp recording. In this method, neuroscientists place a polished glass micropipette against a cell and apply suction to form a high resistance seal. This process isolates a small “patch” of membrane that contains one or more ion channels.
11:12
Hyperinsulinemic-Euglycemic Clamp in the Conscious Rat
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Cited by 34 •
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The hyperinsulinemic-euglycemic clamp is the "gold standard" for the assessment of insulin action. Insulin is infused at a constant rate stimulating glucose uptake. The amount of exogenous glucose infused to counter this drop is indicative of insulin sensitivity. Here the procedure is performed on a conscious, unrestrained rat.
07:38
Patch Clamp Recordings from Embryonic Zebrafish Mauthner Cells
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Cited by 12 •
2013
We have developed an intact brain-spinal cord preparation to record and monitor electrical activity via patch clamp recording from the Mauthner neurons and other reticulospinal cells in zebrafish embryos. Thus, we are able to record excitatory and inhibitory synaptic currents, voltage-gated channel activity and action potentials from key neurons in a developing embryo.
FAQs
The ratchet maintains the selected jaw closure without requiring continuous hand pressure, while serrated jaws help prevent tissue or small structures from slipping. This sustained grip can support controlled separation or temporary compression during a procedure. Because the clamp continues exerting pressure after locking, the operator must match closure to the tissue and intended task rather than relying on force alone.
Jaw placement determines which structure receives pressure and how effectively the clamp can create or maintain a dissection plane. Tissue resistance provides feedback about whether the instrument is meeting expected opposition or applying excessive force. Placing the jaws deliberately and responding to resistance helps practitioners isolate structures while limiting unnecessary trauma to surrounding tissue.
Clamp size should correspond to the tissue or small structure being manipulated and the amount of control required. The overview identifies size and tissue as factors that influence performance, so one clamp choice will not suit every situation. Matching these variables supports secure grasping, separation, or temporary compression without applying more manipulation than the tissue requires.
A controlled sequence begins with deliberate jaw placement around the intended tissue or small structure, followed by gradual closure and engagement of the ratchet when sustained control is needed. The operator then uses the secured position to separate, isolate, or temporarily compress the target. Releasing the ratchet restores jaw movement and permits careful removal after the task is complete.
The technique is useful when a practitioner needs to hold tissue securely while developing a plane or distinguishing one structure from nearby tissue. The clamp can maintain position as the operator works, reducing the need to repeatedly grasp the same area. Its value lies in controlled manipulation that supports separation and identification while limiting unnecessary disturbance of surrounding tissue.
A Kelly clamp can provide temporary compression of a vessel when its jaws are positioned appropriately and the ratchet maintains closure. This gives the operator time to continue working while the vessel remains controlled. The result depends on the clamp size, tissue involved, and applied pressure, making deliberate placement and controlled release important for limiting tissue trauma.