3d Vascular Network Formation
3d Vascular Network Formation - Related Videos
10:53
Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks
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Cited by 10 •
2017
This protocol outlines the implementation of image-guided, laser-based hydrogel degradation to fabricate vascular-derived, biomimetic microfluidic networks embedded in poly(ethylene glycol) diacrylate (PEGDA) hydrogels. These biomimetic microfluidic systems may be useful for tissue engineering applications, generation of in vitro disease models, and fabrication of advanced "on-a-chip" devices.
03:33
Visualization of Neural and Vascular Networks in a Chicken Embryo
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2025
Source: Delalande, J., et.al. Dual Labeling of Neural Crest Cells and Blood Vessels Within Chicken Embryos Using ChickGFP Neural Tube Grafting and Carbocyanine Dye DiI Injection. J. Vis. Exp. (2015)This video demonstrates the transplantation of a GFP-labeled donor neural tube from a stage-matched transgenic chicken embryo into a recipient embryo at the level of somites one to seven, followed by vascular labeling using a lipophilic fluorescent dye. The combined approach allows for direct...
09:38
Scaling of Engineered Vascular Grafts Using 3D Printed Guides and the Ring Stacking Method
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Cited by 6 •
2017
Scalable engineered blood vessels would improve clinical applicability. Using easily sizable 3D-printed guides, rings of vascular smooth muscle were created and stacked into a tubular form, forming a vascular graft. Grafts can be sized to meet the range of human coronary artery dimensions by simply changing the 3D-printed guide size.
09:23
Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration
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Cited by 40 •
2015
In this work, we present a technique for the rapid fabrication of living vascular tissues by direct culturing of collagen, smooth muscle cells and endothelial cells. In addition, a new protocol for the mechanical characterization of engineered vascular tissues is described.
02:26
Generating 3D Co-culture Spheres of Astrocytes and Neurons to Induce Synapse Formation
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2025
This video demonstrates a procedure for co-culturing the astrocytes and neurons to form 3D spheres. These 3D spheres provide a distinct platform for examining synapse formation and neural network integration, thereby serving as a potential model for research in developmental neuroscience, neurodegenerative diseases, and neuropharmacological interventions.
FAQs
These processes contribute at different stages and through distinct starting points. Vasculogenesis builds vessels from vascular progenitor cells, whereas angiogenesis extends and remodels structures from vessels that already exist. Considering both processes helps researchers distinguish initial network assembly from later expansion, restructuring, and maturation within developing three-dimensional tissues.
Endothelial cell migration positions cells within the developing tissue, while proliferation increases the cellular material available for network growth. Lumen formation creates the internal spaces characteristic of vessels, and coordinated remodeling reorganizes the resulting structures. Together, these activities determine how an initially developing vascular arrangement becomes an interconnected network that can support growing tissue.
Vascular patterning shows how vessel arrangements become integrated with organ development and tissue maturation. Examining the distribution and organization of networks in three dimensions can help researchers study how vascular structures relate to the developing tissue environment. This perspective is important because vascular development is connected to the growth and functional progression of organs.
Researchers use three-dimensional culture systems and organoids to model vascular development under controlled conditions. These models provide settings in which network formation, extension, remodeling, and lumen formation can be studied as part of a tissue-like structure. They support developmental biology experiments focused on organ development, tissue maturation, and vascular patterning without relying solely on observations of intact organs.
The topic supports research into congenital vascular disorders, tissue engineering, regenerative medicine, and therapies directed at abnormal vessel growth. Its value comes from linking vessel development with tissue organization and maturation. Three-dimensional models can therefore provide a controlled research context for examining developmental defects, designing tissue-related approaches, and investigating how vascular growth may be altered.
In developmental biology, controlled three-dimensional models allow researchers to examine how vascular networks form and become patterned during tissue development. Comparing normal and abnormal network development can help frame studies of congenital vascular disorders, while organoid systems connect these observations to organ formation and maturation. The same models also provide context for exploring abnormal vessel growth and related therapeutic strategies.