Amniotic Fluid Sampling
Amniotic Fluid Sampling - Related Videos
09:34
Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
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Cited by 3 •
2017
This protocol describes the reprogramming of primary amniotic fluid and membrane mesenchymal stem cells into induced pluripotent stem cells using a non-integrating episomal approach in fully chemically defined conditions. Procedures of extraction, culture, reprogramming, and characterization of the resulting induced pluripotent stem cells by stringent methods are detailed.
08:24
The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System
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Cited by 2 •
2017
In this study, we generate induced pluripotent stem cells from mouse amniotic fluid cells, using a non-viral-based transposon system.
07:36
Modeling Encephalopathy of Prematurity Using Prenatal Hypoxia-ischemia with Intra-amniotic Lipopolysaccharide in Rats
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Cited by 20 •
2015
Encephalopathy of prematurity encompasses the central nervous system abnormalities associated with injury from preterm birth. This report describes a clinically relevant rat model of in utero transient systemic hypoxia-ischemia and intra-amniotic lipopolysaccharide administration (LPS) that mimics chorioamnionitis, and the related impact of infectious stimuli and placental underperfusion on CNS development.
10:23
Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid
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Cited by 2 •
2017
A method for mass spectrometric analysis of endogenous peptides in human cerebrospinal fluid (CSF) is presented. By employing molecular weight cut-off filtration, chromatographic pre-fractionation, mass spectrometric analysis and a subsequent combination of peptide identification strategies, it was possible to expand the known CSF peptidome nearly ten-fold compared to previous studies.
09:58
A Reversible, Non-invasive Method for Airway Resistance Measurements and Bronchoalveolar Lavage Fluid Sampling in Mice
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Cited by 10 •
2010
Repeated measurements of rodent respiratory physiology and sampling of airway inflammatory cells are desirable, but generally not feasible. Here we describe a repeatable method for orally intubating mice that permits repeated measurements of airway hyperreactivity and sampling of airway inflammatory cells.
FAQs
The sample can be examined for several classes of fetal information, including cells, DNA, proteins, and other biological markers. Fetal cells can be cultured for further study, while DNA-based and molecular tests assess genetic material. Protein and biochemical analyses add additional readouts, allowing investigators to examine prenatal development through complementary cellular and molecular evidence.
Ultrasound guidance links the collection step to the developing anatomy by directing the thin needle through the abdominal wall and uterus into the amniotic sac. This guided route enables withdrawal of a small fluid sample for laboratory work. The resulting material can then undergo cellular and molecular analysis for prenatal diagnostic or developmental biology studies.
Cultured fetal cells provide a cellular readout of prenatal development that complements direct analysis of DNA, proteins, and other markers. Once available for laboratory study, they can support diagnosis and help researchers investigate fetal genetic and chromosomal development. In developmental biology, this connects molecular findings with evidence from fetal cells themselves.
After collection, laboratory analysis can use multiple routes rather than a single readout. The sample may undergo examination of fetal cells, DNA-based molecular testing, protein analysis, or assessment of other biological markers. Culturing cells provides an additional analytical pathway. Together, these approaches generate evidence that can support prenatal diagnosis and developmental research.
Findings from amniotic fluid sampling can provide evidence about fetal genetic and chromosomal development, sex-linked conditions, and selected biochemical abnormalities. These categories address different biological questions: genetic and chromosomal analyses examine developmental information in those systems, while biochemical markers provide evidence about biochemical features. Results can contribute to diagnosis and clinical counseling.
Researchers and clinicians use this approach when they need biological evidence about prenatal development that can be examined in the laboratory. In clinical settings, findings may support prenatal diagnosis and counseling. In developmental biology, the same material supports research on genetic and chromosomal development, linking prenatal observations with cellular, molecular, and biochemical measurements.