Colony Splitting
Colony Splitting - Related Videos
10:37
The Split Brain
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2023
Source: Laboratories of Jonas T. Kaplan and Sarah I. Gimbel—University of Southern California The study of how damage to the brain affects cognitive functioning has historically been one of the most important tools for cognitive neuroscience. While the brain is one of the most well protected parts of the body, there are many events that can affect the functioning of the brain. Vascular issues, tumors, degenerative diseases, infections, blunt force traumas, and neurosurgery are just some of the...
06:40
From MEFs to Matrigel 2: Splitting hESCs from MEFs onto Matrigel
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Cited by 4 •
2008
This video demonstrates how to maintain the growth of human embryonic stem cells (hESCs) in feeder cell-free conditions and how to continuously passage hESCs in feeder cell-free conditions. Confirmation of hESC pluripotency grown in feeder cell-free conditions by immunofluorescence microscopy is also demonstrated. Part 2 of 3.
06:44
Keratinocyte Colony Formation Assay: An In Vitro Assay to Assess the Ability of Keratinocytes to Grow into Colonies
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2023
In this video, we perform a clonogenic assay of keratinocytes harvested from adult mice to determine the ability of individual cells to proliferate and form colonies. This keratinocyte harvesting technique is useful for studying other downstream aspects of cutaneous chemical carcinogenesis and tumor promotion.
01:23
Stock Split
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2025
A stock split is a corporate action aimed at increasing the number of a company's shares by dividing its existing shares into multiple new ones. This adjustment reduces the per-share price without altering the company's overall market capitalization, thereby making shares more affordable to investors and enhancing trading flexibility. Stock splits are often employed by companies whose stock prices have risen significantly, making their shares less accessible to retail investors. Lowering the...
01:13
¹H NMR: Complex Splitting
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2024
A proton M that is coupled to a proton X results in doublet signals for M. However, NMR-active nuclei can be simultaneously coupled to more than one nonequivalent nucleus. When M is coupled to a second proton A, such as in styrene oxide, each peak in the doublet is split into another doublet. Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied first.
FAQs
Selection should focus on healthy, undifferentiated regions rather than treating every area of a culture as equivalent. This preserves cells in a state suitable for continued stem-cell maintenance and later developmental experiments. The choice is therefore a biological control point that helps keep the reseeded population aligned with studies of self-renewal, lineage specification, and early tissue development.
A defined reseeding density establishes a controlled starting population for continued culture. It helps researchers provide conditions that support cell attachment and proliferation while making comparisons between cultures more consistent. Because developmental experiments depend on reproducible starting material, controlling density can influence how reliably expanded cells are maintained or prepared for subsequent differentiation studies.
Gentle dissociation separates colonies from the culture surface while supporting the recovery of smaller cell groups for reseeding. The overview identifies mechanical and enzymatic approaches as available options, but emphasizes that the process should remain gentle. This step connects colony removal with the practical goal of establishing new cultures that can attach and continue proliferating.
A typical workflow begins by identifying healthy, undifferentiated colony regions. Researchers then remove those regions from the culture surface through gentle mechanical or enzymatic dissociation, producing cell clumps for transfer. Finally, they reseed the material at a defined density under conditions that support attachment and proliferation, creating a controlled starting point for continued culture or later experiments.
Researchers use this approach when they need to maintain embryonic stem cells or other stem-cell cultures while generating material for new experiments. It supports expansion before differentiation studies and provides cells for investigations of self-renewal, lineage specification, and early tissue development. The technique is especially relevant when experiments require a controlled in vitro population across repeated culture steps.
Consistent handling helps maintain comparable cultured populations from one splitting step to the next. Selecting similar healthy regions, using gentle dissociation, and reseeding at a defined density all contribute to controlled culture conditions. As a result, researchers can improve the reproducibility of stem-cell maintenance and obtain more reliable material for differentiation and developmental biology experiments.