Pollen Grain Examination
Pollen Grain Examination - Related Videos
07:07
A High-Resolution, Single-Grain, In Vivo Pollen Hydration Bioassay for Arabidopsis thaliana
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2023
An improved method to measure pollen hydration profiles in Arabidopsis thaliana is described here. The new method offers higher resolution, is noninvasive, and is highly reproducible. The protocol represents a new tool for a finer dissection of the processes that regulate the early stages of pollination.
06:52
Nanocrystalline Alloys and Nano-grain Size Stability
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2023
Source: Sina Shahbazmohamadi and Peiman Shahbeigi-Roodposhti-Roodposhti, School of Engineering, University of Connecticut, Storrs, CT Alloys with grain size less than 100 nm are known as nanocrystaline alloys. Due to their enhanced physical and mechanical properties, there is an ever-increasing demand to employ them in various industries such as semiconductor, biosensors and aerospace. To improve the processing and application of nanocrystalline alloys, it is necessary to develop close to 100%...
09:51
Atom Probe Tomography Studies on the Cu(In,Ga)Se2 Grain Boundaries
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Cited by 29 •
2013
In this work, we describe the use of the atom-probe tomography technique for studying the grain boundaries of the absorber layer in a CIGS solar cell. A novel approach to prepare the atom probe tips containing the desired grain boundary with a known structure is also presented here.
06:34
A Component-resolved Diagnostic Approach for a Study on Grass Pollen Allergens in Chinese Southerners with Allergic Rhinitis and/or Asthma
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Cited by 4 •
2017
This work describes a protocol that uses a component-resolved approach to study sensitization to grass pollen allergens in a cohort of patients from southern China with allergic rhinitis and/or asthma.
09:13
Characterization of Ultra-fine Grained and Nanocrystalline Materials Using Transmission Kikuchi Diffraction
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Cited by 8 •
2017
This paper provides a detailed method to characterize the microstructure of ultra-fine grained and nanocrystalline materials using a scanning electron microscope equipped with a standard electron backscatter diffraction system. Metal alloys and minerals presenting refined microstructures are analyzed using this technique, showing the diversity of its possible applications.
FAQs
Sampling pollen at defined developmental stages allows researchers to follow the sequence from microsporogenesis through pollen maturation and male gametophyte formation. Comparing grains collected at different points can reveal when cellular organization, wall architecture, or other structural features arise. This stage-based approach is especially useful for identifying developmental abnormalities rather than treating mature pollen as a single uniform population.
Wall architecture, apertures, and cellular organization provide complementary structural information. Wall features and apertures can be compared among developmental stages or plant samples, while cellular organization helps track formation of the male gametophyte. Examining these characteristics together gives a broader picture of pollen maturation than relying on a single visible trait.
Viability and germination assays provide related but distinct evidence about reproductive performance. Viability assessment indicates whether pollen remains capable of functioning, whereas germination testing examines whether it can initiate the process needed to support fertilization. Using both outcomes helps connect microscopic observations with the practical reproductive capacity of the pollen sample.
A basic workflow begins with collecting pollen at selected developmental stages, followed by sample preparation and staining. The prepared grains can then be examined with light microscopy or electron microscopy, depending on the structural information required. Researchers subsequently evaluate features such as wall architecture, apertures, and cellular organization, and may pair these observations with viability or germination assays.
Both methods support structural analysis, but they serve different observational needs within the workflow. Light microscopy is suitable for examining prepared and stained grains, while electron microscopy supports closer analysis of pollen surface or wall architecture. Selecting between them depends on the level of structural detail needed to investigate maturation, abnormalities, or differences among samples.
In developmental biology, the method can clarify how microsporogenesis, pollen maturation, and male gametophyte formation proceed. It can also help identify reproductive abnormalities and relate structural findings to pollen fertility through viability and germination results. Beyond development, researchers apply these observations to plant taxonomy and to studies of environmental effects on reproductive development.