Reusable Molds

Reusable molds are patterned forms designed to produce multiple consistent biological or experimental structures without creating a new mold for each fabrication cycle. They work by shaping a material, such as a polymer, gel, or culture substrate, within defined cavities or channels; after the material sets, the structure is removed and the mold can be cleaned and used again. In developmental biology, reusable molds support the standardized preparation of cell culture environments, tissue constructs, and other model systems that help researchers examine cell organization, growth, and morphogenesis. Their repeatability can reduce fabrication variability, conserve materials, and improve comparisons across experiments.

Reusable Molds - Related Videos

Research

JoVE Journal - Engineering

Reusable Hepatic Imaging Tool: A Reusable 3D-Printed Tool for Hepatic Intravital Microscopy

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2026

This protocol introduces a cutting-edge, Reusable Hepatic Imaging Tool (RHIT) for intravital microscopy (IVM) of live mice, which enhances tissue stability for rapid and acute experiments. The RHIT's 3D-printed design offers cost-effective production, scalability, and versatility, enabling prolonged observation of metabolic processes in mice of various sizes.

Rapid and Low-cost Prototyping of Medical Devices Using 3D Printed Molds for Liquid Injection Molding

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Cited by 34 •

2014

We have devised a method for low-cost and rapid prototyping of liquid elastomer rubber injection molded devices by using fused deposition modeling 3D printers for mold design and a modified desiccator as a liquid injection system.

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

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Cited by 16 •

2016

Presented is the protocol for co-immobilizing whole-cell biocatalysts for cofactor regeneration and improved reusability, using the production of L-xylulose as an example. The cofactor regeneration is achieved by coupling two Escherichia coli strains expressing functionally complementary enzymes; the whole-cell biocatalyst immobilization is achieved by cell encapsulation in calcium alginate beads.

Reusable Single Cell for Iterative Epigenomic Analyses

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2022

The present protocol describes a single-cell method for iterative epigenomic analyses using a reusable single cell. The reusable single cell allows analyses of multiple epigenetic marks in the same single cell and statistical validation of the results.

Production and Use of Customizable Agarose Molds for Scaffold-Free Mouse Ovarian Follicle Culture

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Cited by 2 •

2025

We describe methods to culture ovarian follicles in a novel scaffold-free agarose mold that complement in vitro gametogenesis.

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