Avian Transgenesis
Avian Transgenesis - Related Videos
07:23
Mosaic Zebrafish Transgenesis for Evaluating Enhancer Sequences
0 Views •
Cited by 6 •
2010
We demonstrate our approach to finding potential enhancer elements from developmentally regulated genes and evaluating their function through mosaic zebrafish transgenesis.
08:51
Microinjection for Transgenesis and Genome Editing in Threespine Sticklebacks
0 Views •
Cited by 30 •
2016
Transgenic manipulations and genome editing are critical for functionally testing the roles of genes and cis-regulatory elements. Here a detailed microinjection protocol for the generation of genomic modifications (including Tol2-mediated fluorescent reporter transgene constructs, TALENs, and CRISPRs) is presented for the emergent model fish, the threespine stickleback.
06:57
RNAi Mediated Gene Knockdown and Transgenesis by Microinjection in the Necromenic Nematode Pristionchus pacificus
0 Views •
Cited by 28 •
2011
In model organisms, transgenesis can manipulate gene functions while RNAi can knockdown specific mRNA transcripts 1-2. This protocol aims to illustrate the techniques needed to introduce stably transmitted DNA and transient double stranded RNA into the necromenic nematode Pristionchus pacificus for studies in evolutionary, developmental, and behavioral biology.
01:31
Determining the Minimum Inhibitory Concentration of Enrofloxacin Against Avian Pathogenic E. coli
0 Views •
2026
Begin with a suspension of avian pathogenic E. coli or APEC in growth media.APEC is a Gram-negative bacterium that infects poultry by entering through the respiratory tract.After breaching the mucosal layer, APEC adhere to epithelial cells, multiply locally, and disrupt the epithelial barrier to enter the bloodstream. The bacteria then spread to multiple organs, causing systemic infection.Add an equal volume of the bacterial suspension to each well of a multi-well plate containing increasing...
05:32
Embryo Microinjection for Transgenesis in Drosophila
0 Views •
2024
This article takes the phiC31 integrase-mediated transgenesis in Drosophila as an example and presents an optimized protocol for embryo microinjection, a crucial step for creating transgenic flies.
FAQs
Primordial germ cells are important because they can contribute to the developing embryo and carry an introduced or modified sequence into the germline. If altered germ cells participate in reproduction, the genetic change may be transmitted to offspring rather than remaining limited to one developing bird. This makes them especially valuable for connecting a genetic manipulation with inherited developmental or organismal traits.
Viral vectors and genome-editing tools represent different ways to deliver genetic changes to primordial germ cells or early embryos. The source identifies both as available delivery systems, but does not assign them identical effects or uses. Their shared importance is that they provide access to developmental material before or during embryogenesis, allowing investigators to examine how altered genetic information influences later development.
An altered sequence can be used to test gene function, regulatory elements, cell-lineage decisions, and tissue formation in vivo. These questions connect molecular changes with visible developmental outcomes: whether a gene contributes to a particular process, whether regulatory DNA affects gene activity, how cells adopt different fates, and how tissues emerge during embryonic development. The approach therefore links genetic manipulation to organism-level development.
A typical workflow begins by delivering a transgene or other genetic alteration into primordial germ cells or an early embryo. Modified germ cells can then contribute to embryonic development, after which the resulting bird is evaluated for the altered sequence or associated characteristics. Breeding is used to determine whether the change is transmitted to offspring, establishing its heritable relevance.
Researchers would choose this approach when they need to investigate gene activity and developmental outcomes within a living avian embryo or organism. It is suited to questions about regulatory elements, lineage decisions, tissue formation, and inherited traits. Because the model connects an introduced genetic change with development in vivo, it can complement studies focused only on isolated cells or molecular components.
The approach supports research on avian disease, reproduction, evolution, and the production of birds with precisely altered characteristics. These applications extend the value of developmental models beyond tissue formation: investigators can examine inherited effects relevant to reproductive biology, explore genetic contributions to disease, study evolutionary changes, or assess how targeted alterations influence organismal traits.