Sirna Encapsulation Nanoparticles
Sirna Encapsulation Nanoparticles - Related Videos
03:04
siRNA Encapsulation into Exosomes Using Electroporation: An Electric Pulse Based Technique to Load siRNA into Exosomes
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2023
This video describes an electroporation-based method for encapsulating siRNA into exosomes. The siRNA-loaded exosomes can then be used for therapeutic applications.
03:06
Encapsulating Nerve Growth Factor in High-Density Lipoprotein-Mimicking Nanoparticles
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2025
Source: Zhu, J., et. al., Preparation and Characterization of Novel HDL-mimicking Nanoparticles for Nerve Growth Factor Encapsulation. J. Vis. Exp. (2017)This video demonstrates the stepwise assembly of NGF-loaded HDL-mimicking nanoparticles using protamine-assisted encapsulation and lipid-based self-assembly for potential neurotrophic delivery applications.
08:24
Preparation and Characterization of Novel HDL-mimicking Nanoparticles for Nerve Growth Factor Encapsulation
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Cited by 4 •
2017
Simple homogenization was used to prepare novel, high-density, lipoprotein-mimicking nanoparticles to encapsulate nerve growth factor. Challenges, detailed protocols for nanoparticle preparation, in vitro characterization, and in vivo studies are described in this article.
02:30
siRNA - Small Interfering RNAs
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2020
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements. In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
11:06
Encapsulating Cytochrome c in Silica Aerogel Nanoarchitectures without Metal Nanoparticles while Retaining Gas-phase Bioactivity
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Cited by 1 •
2016
This procedure describes how to encapsulate cytochrome c (cyt. c) in silica (SiO2) sol-gels, process these gels to form bioaerogels, and use these bioaerogels to rapidly recognize nitric oxide (NO) through a gas-phase reaction. This type of protocol may aid in the future development of biosensors or other bioanalytical devices.
FAQs
Particle composition helps determine how effectively the carrier protects siRNA, enters cells, and releases its cargo intracellularly. Release behavior is especially important because siRNA must become available inside the cell to guide silencing activity. Differences in these properties can therefore change delivery efficiency and the strength or consistency of target gene knockdown in experimental models.
Intracellular release places siRNA in the cellular environment where it can guide the RNA-induced silencing complex, or RISC, toward messenger RNA with a complementary sequence. This interaction reduces expression of the selected target gene. If the cargo remains unavailable inside the cell, the intended gene-silencing effect cannot be efficiently produced.
Transient knockdown allows investigators to reduce gene expression without treating the effect as a permanent genetic change. This is useful for testing gene function during developmental processes, including signaling, morphogenesis, and tissue differentiation. The approach can reveal consequences of reduced expression in cells, embryos, or organoid models while supporting functional experiments focused on specific developmental contexts.
A typical workflow begins by packaging the selected siRNA in nanoscale carriers, applying the particles to cells, embryos, or organoids, and allowing cellular uptake and intracellular release to occur. Investigators then examine the resulting reduction in target gene expression and relate that change to developmental features or processes under study.
The approach supports functional studies of genes involved in signaling, morphogenesis, and tissue differentiation. Researchers can apply it in cellular systems, embryos, or organoids to examine how reducing a selected gene changes developmental behavior or tissue formation. These experiments help connect gene expression with developmental outcomes without relying only on descriptive observation.
Optimization focuses on particle composition and the timing or characteristics of intracellular release because both can influence delivery efficiency. Researchers can compare how these properties affect siRNA protection, cellular uptake, and target gene knockdown in the chosen model. Better control of delivery can improve the precision of developmental experiments and clarify gene function.