Bet Isotherm
Bet Isotherm - Related Videos
10:42
Flow Cytometric Analysis of Particle-bound Bet v 1 Allergen in PM10
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Cited by 8 •
2016
Here, we present a protocol to quantify allergen-loaded particles by flow cytometry. Ambient particulate matter particles may act as carriers of adsorbed allergens. We show here that flow cytometry, a method widely used to characterize suspended solids >0.5 µm in diameter, can be used to measure these allergen-loaded particles.
01:21
Isothermal Processes
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2025
A thermodynamic process that occurs at constant temperature is called an isothermal process. Heat slowly flows into the system or out of the system to maintain thermal equilibrium. Processes involving phase changes like water evaporation into steam or freezing water into ice at a constant temperature are examples of Isothermal Processes. An ideal gas can also undergo isothermal expansion or compression. For example, consider 1 mole of an ideal gas inside an isolated cylinder at initial volume V...
02:48
Loop-Mediated Isothermal Amplification for Bacterial Gene Detection
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2025
Source: Domesle, K. J., et al. Loop-Mediated Isothermal Amplification for Screening Salmonella in Animal Food and Confirming Salmonella from Culture Isolation. J. Vis. Exp. (2020).This video demonstrates the loop-mediated isothermal amplification (LAMP) technique for detecting a target bacterial gene by amplifying DNA at a constant temperature. The reaction mixture is prepared by adding an isothermal master mix, specific primers for the target gene, and samples containing DNA templates. The...
01:27
Isothermal Microcalorimetry to Monitor Metabolic Activity in Bacteria
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2025
Take serial dilutions of a bacterial culture and add aliquots into calorimetric vials containing growth medium.Load the sealed vials into the multi-well plate of an isothermal microcalorimeter to measure metabolic heat production.The device is held at a constant temperature to ensure the detected heat originates solely from bacterial metabolism.As bacteria metabolize nutrients and proliferate, they release heat that sensors detect in real time.In parallel, incubate identical vials in an...
08:45
Isothermal Titration Calorimetry for Measuring Macromolecule-Ligand Affinity
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Cited by 84 •
2011
A general protocol for the use of isothermal titration calorimetry to monitor the binding thermodynamics for biological systems with moderate binding affinities is presented.
FAQs
The model treats adsorption as a sequence of equilibrated layers. Gas molecules first occupy surface sites, and molecules in that initial layer provide sites for additional molecules as pressure changes. This multilayer assumption lets the equation represent uptake beyond a single coverage level, making it useful for relating measured adsorption behavior to the accessible structure of a solid.
Langmuir describes adsorption as a single layer, whereas the BET approach extends that framework to sequential multilayer formation. The distinction matters because a solid can continue taking up gas after the first layer is established. In engineering analysis, selecting the multilayer model supports interpretation of adsorption data for materials whose behavior exceeds a one-layer description.
Pressure changes reveal how gas uptake responds as additional layers form, while adsorption-desorption equilibrium supplies the condition assumed by the model. Relating uptake to pressure under this equilibrium framework allows the mathematical treatment to describe layer development. These factors connect the fitted relationship to experimentally observed adsorption behavior in the analyzed solid.
A BET analysis provides a basis for measuring specific surface area and examining related pore characteristics. These descriptors summarize aspects of a solid's accessible surface structure that influence gas adsorption. They allow engineers to compare porous materials and investigate how differences in surface and pore structure relate to performance in practical systems.
An engineer measures nitrogen adsorption while varying pressure, then fits the resulting data to the BET equation. The fit is interpreted to obtain specific surface area and related pore characteristics. Comparing those descriptors among catalysts, activated carbons, powders, or other porous materials helps connect measured surface structure with differences in material performance.
Common targets include catalysts, activated carbons, powders, and other porous materials. Their adsorption responses can reveal differences in accessible surface structure and pore characteristics. Such comparisons are valuable when engineers need to relate material architecture to behavior in separations, reaction systems, or storage technologies across these applications.
Engineering value comes from linking a measurable surface descriptor with system performance. Specific surface area and related pore characteristics can help researchers compare materials used in separations, reaction systems, and storage technologies. This connection supports material selection and performance interpretation, because changes in surface structure may be evaluated alongside the function required from the engineered solid.