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JoVE Journal
Biology

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Isothermal Titration Calorimetry for Measuring Macromolecule-Ligand Affinity
 

Isothermal Titration Calorimetry for Measuring Macromolecule-Ligand Affinity

Article DOI: 10.3791/2796 08:46 min
September 7th, 2011

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Summary September 7th, 2011

A general protocol for the use of isothermal titration calorimetry to monitor the binding thermodynamics for biological systems with moderate binding affinities is presented.

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