Tissue Scaffold
Tissue Scaffold - Related Videos
08:35
Elastomeric PGS Scaffolds in Arterial Tissue Engineering
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Cited by 21 •
2011
Elastomeric PGS scaffolds with vascular smooth muscle cells cultured in a pulsatile flow bioreactor may lead to promising small-diameter arterial constructs with native ECM production in a relatively short culture period.
10:08
Electrospinning Fibrous Polymer Scaffolds for Tissue Engineering and Cell Culture
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Cited by 38 •
2009
The process of electrospinning polymers for tissue engineering and cell culture is addressed in this article. Specifically, the electrospinning of photoreactive macromers with additional processing capabilities of photopatterning and multi-polymer electrospinning is described.
14:49
Self-reporting Scaffolds for 3-Dimensional Cell Culture
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Cited by 7 •
2013
Biocompatible pH responsive sol-gel nanosensors can be incorporated into poly(lactic-co-glycolic acid) (PLGA) electrospun scaffolds. The produced self-reporting scaffolds can be used for in situ monitoring of microenvironmental conditions whilst culturing cells upon the scaffold. This is beneficial as the 3D cellular construct can be monitored in real-time without disturbing the experiment.
04:33
Decellularized Porcine Esophageal Scaffold Preparation: A Technique to Generate Acellular Scaffold from a Pig Esophagus
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2023
In this video, we demonstrate the preparation of a decellularized pig esophagus scaffold, with its biochemical architecture and composition preserved, for tissue engineering applications.
09:43
Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development
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Cited by 17 •
2015
This protocol describes decellularization of Sprague Dawley rat kidneys by antegrade perfusion of detergents through the vasculature, producing acellular renal extracellular matrices that serve as templates for repopulation with human renal epithelial cells. Recellularization and use of the resazurin perfusion assay to monitor growth is performed within specially-designed perfusion bioreactors.
FAQs
Pore size, connectivity, and overall architecture determine how cells attach, migrate, and organize throughout the scaffold. Interconnected pores also support nutrient delivery and waste removal, while creating space for new extracellular matrix deposition. These features therefore affect whether cells remain localized, distribute through the construct, and contribute to tissue formation rather than only occupying its surface.
Scaffold stiffness helps regulate cellular behavior and contributes to the mechanical performance of the developing tissue. The degradation rate must also remain compatible with tissue formation: the framework should provide support while cells deposit new extracellular matrix. Balancing these properties can influence structural stability, replacement by developing tissue, and integration with surrounding tissue during repair.
Natural and synthetic materials provide different options for constructing a scaffold, so selection depends on the intended tissue and design requirements. Material choice affects how the framework supports cell adhesion, mechanical performance, degradation, and extracellular matrix deposition. Engineers can also combine a material with cells or signaling molecules when additional biological guidance is needed.
Design begins by matching scaffold architecture, stiffness, and degradation behavior to the needs of the developing tissue. Engineers also consider whether the construct should include cells, signaling molecules, or both to guide regeneration. These choices are coordinated with requirements for nutrient transport, vascularization, mechanical performance, and integration with the tissue surrounding the repair site.
Tissue scaffolds support engineering strategies for damaged bone, cartilage, skin, and other tissues. Their design can be adapted to the biological and mechanical demands of each target, including the need for cell organization, new extracellular matrix formation, and integration with existing tissue. This makes them useful frameworks for developing tissue replacements and guiding repair.
Researchers can assess whether cells adhere and migrate through the construct, whether nutrients and waste can be transported, and whether new extracellular matrix is deposited. They can also examine mechanical performance, degradation behavior, vascularization, and integration with surrounding tissue. Together, these outcomes indicate how well the scaffold supports tissue formation and functions as a repair framework.