Occupational Air Sampling
Occupational Air Sampling - Related Videos
12:02
Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA
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Cited by 2 •
2018
Determining the fungal diversity within an environment is a method utilized in occupational health studies to identify health hazards. This protocol describes DNA extraction from occupational air samples for amplification and sequencing of fungal ITS regions. This approach detects many fungal species that can be overlooked by traditional assessment methods.
09:16
Air-sampled Filter Analysis for Endotoxins and DNA Content
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2016
Two complementary analyses of atmospheric biological particles from air sampled filters are described herein: the extraction and detection of endotoxin, and of DNA.
01:21
Measuring Cerebral Blood Volume in the Auditory Cortex Using Vascular Space Occupancy
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2025
Position a human participant’s head within the head coil of a functional magnetic resonance imaging, or fMRI, system.Employ vascular space occupancy or VASO, a technique to measure changes in cerebral blood volume, or CBV, the blood volume within the brain.Begin by applying an inversion recovery pulse to suppress signals from blood, allowing only signals from the surrounding tissue within the region to be detected.Next, use MRI-compatible earbuds to deliver auditory stimuli.The stimuli reach...
10:22
Methods to Investigate the Regulatory Role of Small RNAs and Ribosomal Occupancy of Plasmodium falciparum
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2015
Human microRNAs translocate from host erythrocytes to Plasmodium falciparum parasites. Here, the techniques used to transfect synthetic microRNAs into host erythrocytes and isolate all RNAs from P. falciparum are described. In addition, this paper will detail a method of polysome isolation in P. falciparum to determine the ribosomal occupancy and translational potential of parasite transcripts.
09:26
Quantification and Whole Genome Characterization of SARS-CoV-2 RNA in Wastewater and Air Samples
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Cited by 2 •
2023
This protocol aims to quantify SARS-CoV-2 RNA in wastewater and air samples to be used for wastewater-based epidemiology studies and to assess the exposure risk to SARS-CoV-2 in indoor and outdoor aerosols. This protocol also describes a tiled amplicon long-template sequencing approach for SARS-CoV-2 whole genome characterization.
FAQs
The sampling period determines what exposure pattern the result represents. A task-based sample can focus on a particular operation, whereas a shift-based sample characterizes conditions across a work period; location-based sampling adds information about where airborne contaminants occur. Recording the defined period and place helps relate measurements to workplace activities and environmental health risks.
Collection media determine how airborne contaminants are captured for later identification or quantification. Filters, sorbent tubes, and direct-reading instruments provide different measurement pathways, so the selected device should match the information the monitoring program needs. This distinction matters because results may support detailed contaminant assessment or measurement using an instrument rather than collected media.
Exposure limits provide the benchmark for interpreting measured concentrations, but the comparison is meaningful only when the sampling period and workplace context are clearly defined. Relating results to applicable limits helps determine whether existing conditions warrant attention and supports decisions about ventilation, personal protective equipment, or work practices. Measurement therefore becomes part of a control-assessment process.
Occupational air sampling can examine a single worker task, an entire shift, or a workplace location, while environmental health interpretation considers the broader significance of those airborne conditions. Combining these perspectives helps reveal exposure patterns and connects measurements with prevention strategies. The approach is useful for individual monitoring decisions as well as wider workplace monitoring programs.
A practical workflow begins by defining the task, shift, or location to be characterized, then selecting an appropriate sampling device and collection medium. Air is drawn through the chosen system in a measured volume, after which contaminants are identified or quantified. The resulting measurements can then be compared with applicable exposure limits and used to assess controls.
Monitoring programs use these measurements to evaluate whether workplace controls are addressing airborne hazards. When results indicate a need for improvement, findings can guide changes in ventilation, personal protective equipment, or work practices. Sampling across relevant tasks, shifts, or locations also helps characterize exposure patterns and supports prevention strategies and regulatory decision-making.
Results can reveal exposure patterns rather than only a single contaminant value. Organizing measurements by task, shift, or location helps connect airborne conditions with workplace activities and environmental health risks. This context supports hazard assessments, informs monitoring-program design, and provides evidence for prevention strategies or regulatory decisions when organizations need to improve how exposures are understood and managed.