Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Environment / Quantification and Whole Genome Characterization…
JoVE Journal
Environment

A subscription to JoVE is required to view this content.

JoVE Journal Environment
Quantification and Whole Genome Characterization of SARS-CoV-2 RNA in Wastewater and Air Samples
Specific and Accurate Detection of the Citrus Greening Pathogen Candidatus liberibacter spp. Using Conventional PCR on Citrus Leaf Tissue Samples
Journal (Environment)
Specific and Accurate Detection of the Citrus Greening Pathogen Candidatus liberibacter spp. Using Conventional PCR on Citrus Leaf Tissue Samples
Reproducible dsRNA Microinjection and Oviposition Bioassay in Mosquitoes and House Flies
Journal (Environment)
Reproducible dsRNA Microinjection and Oviposition Bioassay in Mosquitoes and House Flies
 

Quantification and Whole Genome Characterization of SARS-CoV-2 RNA in Wastewater and Air Samples

Article DOI: 10.3791/65053-v • 09:26 min • June 30th, 2023
June 30th, 2023 •
1,2,3, 4,5,6, 3, 3, 1,2, 1,2, 1,2, 2,3, 4,5, 1,2
1Institute of Sustainable Processes, University of Valladolid, 2Department of Chemical Engineering and Environmental Technology, University of Valladolid, 3Department of Public Health Microbiology, National Laboratory of Health, Environment and Food, 4ICBAS - School of Medicine and Biomedical Sciences, Porto University, 5Epidemiology Research Unit (EPIUnit), Instituto de Saúde Pública da Universidade do Porto, 6Laboratório para a Investigação Integrativa e Translacional em Saúde Populacional (ITR)

Chapters

Summary
Automatic Translation

This protocol aims to quantify SARS-CoV-2 RNA in wastewater and air samples to be used for wastewater-based epidemiology studies and to assess the exposure risk to SARS-CoV-2 in indoor and outdoor aerosols. This protocol also describes a tiled amplicon long-template sequencing approach for SARS-CoV-2 whole genome characterization.

Tags

Quantification Whole Genome Characterization SARS-CoV-2 RNA Wastewater-based Epidemiology Viral RNA COVID-19 Control Strategies Potential Outbreaks Population-wide Snapshot Asymptomatic Cases Air Sampling Clinical Diagnostics Disease Outbreaks Pandemics Molecular Biology Mengovirus Centrifugation Ultrafiltration Device RNA Extraction Air Samples
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter