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Methods to Investigate the Regulatory Role of Small RNAs and Ribosomal Occupancy of Plasmodium falciparum
Methods to Investigate the Regulatory Role of Small RNAs and Ribosomal Occupancy of <em>Plasmodium falciparum</em>
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Methods to Investigate the Regulatory Role of Small RNAs and Ribosomal Occupancy of Plasmodium falciparum

Methods to Investigate the Regulatory Role of Small RNAs and Ribosomal Occupancy of Plasmodium falciparum

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10:22 min

December 04, 2015

DOI:

10:22 min
December 04, 2015

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Summary

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Human microRNAs translocate from host erythrocytes to Plasmodium falciparum parasites. Here, the techniques used to transfect synthetic microRNAs into host erythrocytes and isolate all RNAs from P. falciparum are described. In addition, this paper will detail a method of polysome isolation in P. falciparum to determine the ribosomal occupancy and translational potential of parasite transcripts.

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