Gallbladder Dissection
Gallbladder Dissection - Related Videos
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The gallbladder is a small, pear-shaped organ that plays a crucial role in our digestive system. Measuring about 10 cm in length, it is comparable in size to a kiwi fruit and is located in a hollow area on the lower surface of the liver. The gallbladder's primary function is to store and concentrate bile, a fluid produced by the liver that aids in digestion. The gallbladder's anatomy consists of three regions: the fundus, body, and neck. Extending from the neck, the cystic duct joins the common...
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Liver and gallbladder diseases are a significant health concern, with prominent conditions including cirrhosis, hepatitis, non-alcoholic fatty liver disease (NAFLD), and gallstones. Jaundice is a common manifestation of liver and biliary disease. Cirrhosis is characterized by the scarring of hepatic lobules in the liver, which are replaced by fibrous tissue, affecting the liver's normal functioning. NAFLD, on the other hand, is caused by an excessive build-up of fat in the liver, not related to...
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This protocol describes a procedure for identifying and dissecting organs from the adult zebrafish.
07:40
Dissection of the Adult Zebrafish Kidney
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The zebrafish kidney is home to both renal and hematopoietic adult stem/progenitor cells, and represents an outstanding opportunity to study these cell types and their progeny in a vertebrate model organism. Here, we demonstrate a detailed dissection procedure that enables the researcher to identify and surgically remove the adult zebrafish kidney, which can be used for applications such as cell isolation, transplantation, and expression studies of kidney and/or blood cell populations.
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Drosophila Larval NMJ Dissection
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This protocol demonstrates how to dissect Drosophila larvae in preparation for immunohistochemistry and/or imaging of the neuromuscular junction.
FAQs
Layer-by-layer examination separates the serosa, muscular wall, mucosa, lumen, and bile as distinct observations rather than treating the organ as a single structure. Comparing these regions helps investigators relate visible tissue organization to gallbladder function and identify where abnormalities such as wall thickening or lesions are located.
Preserving thickening, obstruction, and lesions maintains the tissue changes that may indicate disease-related damage. Their location and appearance can then be considered alongside the gallbladder wall, lumen, bile, and biliary connections. This supports more meaningful pathology assessment than an examination that removes or disrupts abnormal features before they are documented.
The biliary connections place observations from the gallbladder within the wider pathway associated with bile storage and release. Examining these connections with the lumen and bile can help relate an apparent obstruction or other lesion to the organ’s tract relationships. That context is important when assessing structural changes linked to cholecystitis or other abnormalities.
The organ is opened in a controlled, layer-by-layer manner, allowing examination of the serosa, muscular wall, mucosa, lumen, and bile. The procedure also includes assessment of the biliary connections and preservation of notable findings, including thickening, obstruction, or lesions. These observations create a direct anatomical and pathological record for later interpretation.
This approach is useful when investigators need to characterize inflammatory responses, tissue damage, or possible microbial involvement in the biliary system. In conditions such as cholecystitis, direct examination connects these research questions with localized anatomical findings. It therefore provides tissue-level context for studying host–pathogen interactions rather than relying only on general disease descriptions.
Findings can support three complementary outcomes: anatomical education, pathology assessment, and investigation of host–pathogen interactions. Anatomical observations clarify tissue organization, while preserved abnormalities provide material for evaluating disease-associated changes. In infection-focused work, the same examination can help relate inflammatory or damaging features to microbial involvement within the biliary system.