Lung Recruitment Maneuver
Lung Recruitment Maneuver - Related Videos
04:28
Lung Tumor Cell Recruitment Assay
0 Views •
2019
This manuscript describes a method to quantify tumor cell accumulation in the lungs in an animal model of tumor metastasis.
05:52
An In Vitro Flow Investigation of the Effect of Stromal Cells on Leukocyte Recruitment
0 Views •
2025
The video demonstrates a method for investigating the impact of stromal cells on leukocyte recruitment. In a parallel plate flow chamber model, pro-inflammatory cytokines induce the expression of adhesion receptors by endothelial cells, facilitating neutrophil recruitment and migration. The cross-talk between stromal cells and endothelial cells diminishes neutrophil recruitment to the endothelial cells.
11:30
Analyzing the Effects of Stromal Cells on the Recruitment of Leukocytes from Flow
0 Views •
Cited by 28 •
2015
The ability of inflamed endothelium to recruit leukocytes from flow is regulated by mesenchymal stromal cells. We describe two in vitro models incorporating primary human cells that can be used to assess neutrophil recruitment from flow and examine the role that mesenchymal stromal cells play in regulating this process.
04:29
Monitoring Neutrophil Recruitment Dynamics and Bacterial Burden at the Infection Site in a Murine Model
0 Views •
2025
This video demonstrates in vivo imaging to study the neutrophil recruitment dynamics at an infection site in an immunocompromised, transgenic mouse with GFP-expressing neutrophils. It involves injecting luminescent bacteria to cause an infection. This leads to neutrophil recruitment to the infection site, whose tracking is done by imaging.
07:06
A Simple Method of Mouse Lung Intubation
0 Views •
Cited by 50 •
2013
This paper describes a striaghforward and efficient method of intubating mice for pulmonary function measurements or pulmonary instillation, that allows the mice to recover and be studied at later times. The procedure involves an inexpensive fiberoptic light source that directly illuminates the trachea.
FAQs
The maneuver uses a controlled rise in inspiratory pressure to increase the pressure driving air into regions that have collapsed or become poorly aerated. This can restore part of the functional lung volume and improve gas exchange when atelectasis contributes to impaired respiratory function. The pressure increase must remain controlled because excessive force may overdistend already open alveoli.
PEEP, or positive end-expiratory pressure, helps maintain the newly improved level of aeration after the recruitment phase ends. Without an appropriate end-expiratory pressure, alveoli may lose stability and become poorly aerated again, a process called derecruitment. Selecting an appropriate level therefore supports the persistence of improved lung volume rather than only producing a temporary response.
The expected benefit depends on whether atelectasis is contributing to reduced respiratory function and whether the patient can tolerate higher airway pressures. Pressure that is too high can overdistend lung tissue, impair hemodynamics, or contribute to ventilator-associated injury. Patient selection, controlled pressure changes, and observation of the response are therefore central to balancing recruitment against these hazards.
Monitoring should focus on the patient’s respiratory response and tolerance as airway pressure is increased and afterward. Clinicians assess whether oxygenation and gas exchange improve while watching for signs of pressure-related harm, including overdistension or hemodynamic compromise. The maneuver should be conducted as a controlled intervention, followed by an appropriate PEEP level and continued reassessment for renewed derecruitment.
Clinicians may consider this approach during mechanical ventilation when atelectasis is associated with impaired respiratory function or reduced oxygenation. Its purpose is to support restoration of functional lung volume and gas exchange, not to treat every cause of respiratory impairment. Careful patient selection is necessary because the potential benefit must be weighed against pressure-related complications.
A favorable response may include improved oxygenation, better gas exchange, and restoration of functional lung volume when collapsed or poorly aerated regions were limiting respiratory performance. These findings must be interpreted alongside patient tolerance and the durability of the response after PEEP is applied. Lack of improvement, overdistension, or hemodynamic compromise indicates that the intervention requires careful reconsideration.