Porcine Tibial Allotransplantation Model
Porcine Tibial Allotransplantation Model - Related Videos
10:31
Surgical Angiogenesis in Porcine Tibial Allotransplantation: A New Large Animal Bone Vascularized Composite Allotransplantation Model
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Cited by 5 •
2017
Currently any kind of vascularized composite allotransplantation depends on long-term-immunosuppression, difficult to support for non-life-critical indications. We present a new porcine tibial VCA model that can be used to study bone VCA and demonstrate the use of surgical angiogenesis to maintain bone viability without the need of long-term immune-modulation.
04:42
Generating a Rat Model of Tibial Neuroma Transposition
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2025
Source: Brakkee, E. M., et al. Surgery and Behavioral Testing in the Tibial Neuroma Transposition Model in Rats. J. Vis. Exp. (2023).This video demonstrates a procedure to generate a rat tibial neuroma transposition (TNT) model. After exposing the tibial nerve in the hind leg of an anesthetized rat, transect the nerve. Next, create a subcutaneous tunnel, thread the severed nerve through the tunnel, and suture it in place. In the absence of a target for reinnervation, disorganized axonal...
07:23
Surgery and Behavioral Testing in the Tibial Neuroma Transposition Model in Rats
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Cited by 2 •
2023
This protocol describes the tibial neuroma transposition model, which entails a lesion of the tibial nerve with subsequent transposition of the proximal nerve end toward a subcutaneous pretibial or lateral position. Behavioral testing of neuroma pain and plantar hyperalgesia is quantified using Von Frey monofilaments.
09:56
A Rat Model of Tibial Cortex Transverse Transport for the Treatment of Lower Limb Ischemia
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Cited by 1 •
2026
This procedure demonstrates tibial cortex transverse transport in rats, enabling visualization and analysis of revascularization during limb ischemia.
05:06
Tumor Allotransplantation in Drosophila melanogaster with a Programmable Auto-Nanoliter Injector
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Cited by 10 •
2021
This protocol provides detailed guidance for the initial and continued generational allotransplantation of Drosophila tumors into the abdomen of adult hosts for studying various aspects of neoplasia. Using an autoinjector apparatus, researchers can achieve improved efficiency and tumor yields compared to those achieved by traditional, manual methods.
FAQs
Genetic distinction creates an experimental setting in which the recipient can recognize the implanted tibial tissue as biologically different. That response allows investigators to examine immune recognition and rejection mechanisms in living tissue rather than in an isolated assay. The model is therefore useful for testing whether immunosuppressive strategies alter graft acceptance and support successful bone healing.
Investigators can follow several connected outcomes: graft incorporation, revascularization, bone remodeling, and immune recognition. Incorporation indicates how the transplanted segment becomes integrated with recipient tissue, while revascularization reflects restoration of blood supply. Remodeling shows structural adaptation over time. Evaluating these outcomes together helps distinguish healing-related changes from immune-mediated graft injury.
Porcine bone provides a physiologically relevant platform because its size, structure, and healing responses resemble important aspects of human orthopedics. This similarity supports assessment of surgical reconstruction and transplantation protocols under conditions that better reflect orthopedic practice than many smaller experimental systems. Findings can consequently inform treatment development before potential clinical application.
The model permits comparison of graft responses in the presence of different immunosuppressive approaches while maintaining the biological complexity of a living recipient. Researchers can relate treatment exposure to immune recognition, rejection, graft incorporation, revascularization, and remodeling. This integrated assessment helps determine whether an approach protects the graft without overlooking structural healing outcomes important to transplantation and reconstruction.
A study begins with separate donor and recipient pigs, followed by removal of a tibial segment from the donor and implantation of that segment into the recipient. Subsequent evaluation focuses on graft incorporation, blood-vessel restoration, bone remodeling, and immune recognition. Depending on the research question, the protocol can also examine graft preservation or immunosuppressive treatment.
Researchers would choose this system when they need to study bone transplantation, rejection, graft preservation, immunosuppression, or surgical reconstruction in a large-animal setting. Its relevance is especially useful for evaluating transplantation protocols and treatments before clinical application. The model connects immune and skeletal outcomes, making it suitable when both graft survival and orthopedic healing matter.