Premixed Mta

Premixed MTA (mineral trioxide aggregate) is a ready-to-use, calcium silicate-based dental biomaterial used to seal and repair defects in teeth, reducing the need for chairside powder-liquid mixing. When exposed to moisture, its cement particles hydrate to form calcium silicate hydrate gel and calcium hydroxide, producing an alkaline environment and a solid seal. In endodontic and restorative procedures, clinicians apply it for direct pulp capping, perforation repair, root-end filling, and management of open apices. Its moisture tolerance, sealing ability, and potential to support hard-tissue formation make premixed MTA useful when maintaining tooth structure and preventing microbial leakage are clinical priorities.

Premixed Mta - Related Videos

Research

JoVE Journal - Bioengineering

Point-of-care CRISPR-based Diagnostics with Premixed and Freeze-dried Reagents

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Cited by 9 •

2024

Here, we present the step-by-step preparation of premixed, lyophilized recombinase-based isothermal amplification and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-based reactions, which can be used for the detection of nucleic acid biomarkers of infectious disease pathogens or other genetic markers of interest.

In Vitro Imaging and Quantification of the Drug Targeting Efficiency of Fluorescently Labeled GnRH Analogues

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Cited by 1 •

2017

Selectively labeled fluorescent GnRH-I, -II and -III derivatives are reliable tools for tracking and quantifying their cellular uptake. This manuscript introduces experiments to visualize, quantify and compare the uptake efficiency of these GnRH conjugates in various cell lines.

Quantitative Polymerase Chain Reaction to Enumerate Bacteriophages

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2025

In this video, we discuss the quantitative polymerase chain reaction, qPCR, to enumerate T7 bacteriophages through DNA quantification. The fluorescent dye in the PCR mixture binds with the newly synthesized DNA strands and emits fluorescence, which is measured.

Research

JoVE Journal - Immunology and Infection
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A Multiplex Serological Assay for the Detection of Antibody Responses to Arboviruses

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2025

Multiplex fluorescence immunoassays are becoming a reference for serology testing. Their application, however, is limited by the complexity and time-consuming bead-analyte coupling processes. This report presents a semi-automated, reproducible multiplex microsphere bead coupling protocol that is comparable to the standard method while considerably reducing time to results.

Simple Bulk Readout of Digital Nucleic Acid Quantification Assays

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Cited by 5 •

2015

We describe an endpoint digital assay for quantifying nucleic acids with a simplified (analog) readout. We measure bulk fluorescence of droplet-based digital assays using a standard qPCR machine rather than specialized instrumentation and confirm our results by microscopy.

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